Vol.8 (2018) No. 3 ISSN: 2088-5334 Responses Growth and Yield of Three Shallot Cultivars in Sandy Coastal Land with PGPR (Plant Growth Promoting Rhizobacteria) Sumiyati Tuhuteru # , Endang Sulistyaningsih # , Arif Wibowo * # Department of Agronomy, Faculty of Agriculture, Gadjah Mada University, Yogyakarta, 55281, Indonesia * Department of Plant Diseases, Faculty of Agriculture, Gadjah Mada University, Yogyakarta, 55281, Indonesia E-mail: tuhuteru.umy@gmail.com Abstract— Plant Growth Promoting Rhizobacteria (PGPR) is a group of bacteria rhizosphere used to a biological fertilizer on agricultural activities. The purpose of this research got the kind of PGPR which was effective against the growth of three cultivars at sandy coastal land. The research was conducted from August - November 2015 in coastal sandy land Samas-Bantul. This research used a randomized block design (RCBD) with two factor such us cultivar of shallot and suspension PGPR. The first factors were the Crok cultivar, Tiron, and Tuk-tuk and the second factors were Control (without PGPR), PGPR isolates BP25.2 (Bacillus methylotrophic), BP25.6 (Bacillus amyloliquofaciens), BP25.7 (Bacillus subtilis), BrSM 4 (Burkholderia cepacian), and BrSG 5 (Burkholderia seminalis). Data were analyzed by using ANOVA α 5%. If there were a significant difference between treatments, so it was analyzed by DMRT α 5%. Results showed significant through interactions every variable generated, such as isolates BP25.2 that can enhance seed vigor index Tiron cultivars, isolates BrSG 5 and BP25.7 capable of inducing number of leaves in the vegetative phase and is capable of stimulating content proline in the face of stress shallot plant at sandy coastal, isolates BrSM 4 is able to increase the number of total chlorophyll Tuk-tuk cultivars and isolates BrSG 5 is able to increase the dry weight of bulbs production Tuk-tuk cultivars. Keywords— shallots; PGPR; sandy coastal land I. INTRODUCTION Shallots are vegetable crops, which have long been intensively cultivated by farmers. The main producing provinces are marked with shallot harvest area exceeding a thousand hectares per year are North Sumatra, West Sumatra, West Java, Central Java, Yogyakarta, East Java, West Nusa Tenggara, Central Sulawesi and South Sulawesi. These eight provinces accounted for 96.8% of the total shallot production in Indonesia in 2013. Meanwhile, the five provinces in Java Island which consist of West Java, Central Java, Yogyakarta, East Java, and Banten are contributed 78.1 % of the total shallot yield nationwide. Average consumption of shallots per capita for 2011-2012 ranged from 2.36 kg/year and 2.74 kg/year [2]. Shallots are spread in almost all parts of Indonesia. In 2015, exports of shallot soared 219%. Correspondingly, shallot imports fell 82% in the same period. Based on data from the Ministry of Agriculture [2], national shallot yield in 2015 reaches 1.265 million tons, while the demand is only 947.385 ton. Thus, the national shallot production surplus is 318.325 ton, [2]. Therefore, in order to maintain the results achieved, the use of marginal lands, as the coastal sandy need to improve and then alternative substitution of manure [13], as PGPR. Along with the use of biological fertilizers, combining with fertilizer use organic materials known very well be used to improve the physical and biological properties of soil, improve the effectiveness of soil microorganisms and more friendly to the environment [17]. Plant Growth Promoting Rhizobacteria (PGPR) is one of a group bacteria rhizosphere biological fertilizer used in agriculture. PGPR is one of the microbes using alternative chemical fertilizer [8]. In addition, PGPR application usage is expected to increase the growth and productivity of shallot crop cultivated in the sandy coastal land, so constraints the land in farming can be minimized [11]. PGPR is known for its ability to provide and mobilize or facilitate the absorption of various nutrients in the soil as well as synthesizing and changing the concentration of various phytohormone hyper growth. In addition, its functions related to the ability to 849