1 Supplementary Information A new angle on dynamic depolarized light scattering: number-averaged size distribution of nanoparticles in focus Christoph Geers a , Laura Rodriguez-Lorenzo a , Dominic Urban a , Calum Kinnear a,b,c , Alke Petri-Fink a,b , Sandor Balog a * a Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland b Chemistry Department, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland c Institute & School of Chemistry, The University of Melbourne, 30 Flemington Rd – 3010, Australia Table of Content Correlation function of depolarized scattering from polydisperse nanoparticles ........................................................... 2 Influence of polydispersity on DDLS spectra ..................................................................................................................................5 The lognormal distribution....................................................................................................................................................................7 Z-average .......................................................................................................................................................................................................8 TEM versus light scattering....................................................................................................................................................................8 Nanoparticle tracking analysis .......................................................................................................................................................... 10 Taylor-Aris dispersion .......................................................................................................................................................................... 11 Depolarized scattering from multimodal suspensions ............................................................................................................ 13 Small-angle X-ray scattering and TEM of PEG-coated Au NPs ................................................................................................ 14 Correlation function of depolarized scattering from polymer-coated particles ............................................................ 15 Raw moments of P( ) ............................................................................................................................................................................ 16 UV-Vis spectra of gold citrate nanoparticles incubated in BSA solution ........................................................................... 16 Light scattering from nanoparticles in biological media......................................................................................................... 16