Nanostructured layers from DNA, DNA:AU, DNA:C 60 clusters O. Ivanyuta * , D. Kolomiyets, O. Prokopenko, O. Schaternik Radiophysical Faculty and the Scientific and Training Center ‘‘Physical and Chemical Material Science’’ of Kiev National Taras Shevchenko University, 64, Vladimirskaya str., Kiev 01033, Ukraine Abstract The idea is requisite to coat DNA, DNA:Au, DNA:C 60 clusters from water solution, which can be magnetic and electrical active in biosensor systems and to detect their functional properties by microwave techniques (Melkov, G.A., Egorov, Y.V., Ivanyuta, A.N.,Malyshev, V.Y., Zeng, H.K., Wu, K.H., Juang, J.Y., 2000. J. Supercond. 13 (1), 95). Our research has been focused on the application of I–V characteristics and surface microwave resonator methods to recognise and predict these molecular interactions based on primary structure and associated physic-chemical properties. In results we have actually shown that these molecular cluster layers on Si and Al 2 O 3 substrates can conduct, switch electric current and respond on power of microwave (additives Au, C 60 , determine the conductivity of layers). We also aim to apply these Si and Al 2 O 3 ships for Biochip. # 2006 Published by Elsevier B.V. Keywords: Biochip; Al 2 O 3 ; Cluster layers 1. Introduction and state of the problem The aim of this work was an investigation caused by functional properties DNA, DNA:Au, DNA:C 60 clusters from water solution for sponsorship building biochip. Since the electroactivity in nucleic acids was discovered at the beginning of the sixties (Palecek, 1960), different electrochemical tools for DNA based diagnostics and other areas of biotechnology have been performed for detecting DNA, DNA:Au, and DNA:C 60 substructures to analyze or quantificate of optical and electrical active in biosensor systems (Buzaneva and Scharff, 2004). Immediate applications will include directly quantifying DNA, DNA:Au, and DNA:C 60 samples for use in sequencing or polymerase chain reactions (PCR), or pharmaceutical testing and quality control. Besides, it is also expected to study caused by quantum effects features of these phenomena at low temperature. Eventually, they could be applied to producing credit card-sized sensor arrays for clinical applications such as detection of pathogenic bacteria, tumors, and genetic disease, or for forensics. As it is known there are phenomena occurring in such systems are fundamental to the working of synthesized molecular devices, systems but also to living organisms. The ability of nanosystems creation is essential to introduce a wide range of chemical and materials flexibility into these structures to build up more complex nanostructures that can ultimately rival with biological nanosystems. In this respect, DNA, DNA:Au, and DNA:C 60 (Adessi et al., 2003) and MPc/fullerene are potentially ideal nanoscale building blocks because of their length scale, well-defined architecture, controlled technologies, ease of processing and wide range of chemical functionality that can be incorporated. The variety of alive organisms has appeared, evolutional and now exists due to persistent interaction with various factors of environment, adapting to their influence and changes, using them in the vital functions. The majority of these factors have electromagnetic nature. For the spectrum range, where hn > kT , all the kinds of the biological activity to a certain degree have been already found. The case is somewhat different with the rest of wide range of electromagnetic spectrum, where hn < kT . This range includes diapasons from the superhigh frequency (SHF) to infralow frequency (ILF). For a long time ILF range is considered not to influence on alive organisms. The simple physical considera- tions led for such conclusions: as energy quantum in the spectrum range considerably less, from than the average kinetic energy of molecules (hn  kT), then ILF absorption in alive tissue may be associated only with the amplification of a molecule rotation, i.e. with the transformation of electromagnetic energy into thermal one (Grunberg and Keilmann, 1983). www.elsevier.com/locate/geneanabioeng Biomolecular Engineering 24 (2007) 141–142 * Corresponding author. Tel.: +380 44 266 05 32; fax: +380 44 266 06 00. E-mail address: mga@univ.kiev.ua (O. Ivanyuta). 1389-0344/$ – see front matter # 2006 Published by Elsevier B.V. doi:10.1016/j.bioeng.2006.05.024