162 Poster Sessions project voiced HIPAA-based concerns about their patients par- ticipating in the study. Although iterations of the Privacy Rule (December, 2000; August, 2002) allowed for disclosure of PHI without individual authorization for research purposes (Waivers of Authorization), these provisions requrred time for mter- pretataolL implementation and dissemination within research and non-research communities. Given these observations and in order to mimimze the number of future laboratory/surgeon refusals, subject identification and recruiknent were, by choice, severely curtailed. In August, 2003 laboratory recruitment was reinstated with more positive acceptance by laboratories and surgeons. Conclusion: HIPAA concerns continue; however, with tile passage of time and research-related refinements in the Privacy Rule, study protocols using PHI for purposes of identifying, contacting and recrultmg subjects are still viable. tumor. Radiographs were performed using close-up monitoring system (SOFTEX Model CMBW-2, Tokyo Japan). Results: In this study, we successfully established an excellent tumor model in rat using k-ras-expressing RK3E ceils. Tumor masses can be observed as early as 3 days aRer Injection of cells m both subcutaneous and oral mucosa. Tumor size and volume were increased exponentially for 2 weeks. Subcuta- neously injected rats showed minor invasion in the peritoneal cavity within 15 days. RK3E-k-ras cells in oral mucosa also promote morphologic tumor transformation, cell growth and invasion. Maxillary bone Invasion was observed within 12 days. Conclusion: We developed rapid, easy-to-ttse, and cost- effective rat animal model using k-ras overexpressing RK3E cell lines. This tumor animal model has great potential for studying cancer processes and short-term screening of variable cancer therapy strategy. 1•4• Risk factors of laryngeal cancer in patients attended in UNESP of Aragatuba, Brazil M.C. Golato, A.U.R. Fernandes, D.M. dos Santos, M.LMM. Sundefeld. UNESP - S~o PauIo State Unwerstt); Arafatuba Campus, Braztl Introduetion: Patients subrmtted to total laryngectomy have as result from surgacal resection the transfer of the atrways fuac- tlon to the tracheostomal neck openang. There are techmques developed for the manufacmnng of a tracheostoma aesthetic prosthesis from the molding and manufacturing of a acrylic resin tube and, as result, patients feel more comfortable about thetr social hfe. Tile aam of tins study is to perform a survey on data related to the Incidence of laryngeal cancer that would enable ats analysas anlong pataents wath larya~x cancer attended between 1991 mad 2003 at the Oral Oncology Center from the Dentastry School - UNESR Aragatuba. Materials and Methods: Information of gender, age, race, tobacco mad alcohol use and occupational history were obtained from patients through a standardized questloimatre. Results: The largest number of cases of laryngeal cancer occurred among farmers, building constructions workers and drwers. The incidence was higher among men, smokers, white individuals with ages ranging from 50 to 60 years. Conelusion: Based on the results obtained, one may conclude that the laryngeal cancer is dependent on tame and on the exposition to risk factors, also occurring due to the effect of the SOClO-econoinac status, daet, Income and educational level. I• Rapid Induction of Solid and Oral Tumor in Sprague Dawley Rat by Injection of RK3e-ras Cells S.-G. Ahn, H.-W. Kin1, "L-C. Chon, S.-A. Klm, J.-H. Yoon. Department of Oral Pathology, School of Denttst~y. Chosun Unwers~ty, Gwcmglu, Korea Introduetion: Recently, increased interest and efforts were devoted to develop methods for transductlon and formation of rumors In anmml. Here we tried to develop rapid, easy- to-use, and cost-effective rat anmml model of mvaslve cancer which can be readily applied to studying cancer processes and therapy. Materials and Methods: Three-week-old male Sprague Daw- ley rats were shaved and rejected subcutaneously with a single dose of k-ras-expressmg RK3E cells. Cells also injected into the oral mucosa or tall veto. The animals were observed for tu- mor growth and metastasis. Tumor volume (V) was calculated by the formula determined by Carlsson: V = 1/2(ab2), m which 'a' is the longest diameter and 'b' is the shortest diameter of the [• Bayesian geographical analysis at county level on oropharyngeal cancer mortality in the United States, 1969-2001 B. Ettore. Eptdemtology Umt, Centro dt R~fertmento Oncologtco, Avmno, Italy Introduction: Oropharyngeal cancer is linked to known risk factors. A descriptive geographical and temporal analysis on oral cancer mortahty was carried out m the Umted States at county level to update the reformation on this topic. Materials and Methods: Death certification and population data were obtained from SEER mortality database. Data were subdivided by age (5-year age groups), gender, comity of residence, and period (1969-71 and 1999-2001). Hierarchical Bayesian disease-mapping techniques were used to display and stablhse Relative Risk (RR) estimates based on small numbers at county level. Statistical significance of clusters was indicated by 95°,'0 Crediblhty Interval. Results: During the study period, age-standardised (world pop- ulatlon) mortality rates nearly halved among white nien (from 4.73/100,000 In 1969 1971 to 2.49/100,000 in 1999~001) and dechned from 1.47 to 0.91 among white females. Among black males rates decreased shghtly from 6.48 to 5.17 and m females from 1.82 to 1.29. At geographical level, during 1969-1971, both white males and females displayed statisti- cally slgmficant clusters (RR > 10%) of rnortahty along the eastern seaboard and m California, while anaong males only excesses were observed in scattered metro counties of Great Lakes area. Among black males excesses were displayed in the northeastern comatles (mainly metro areas) and m Florida. In recent years (1999-2001), geographic mortahty varmtlon was less pronounced with higher rates, although not statlstmally significant, among white and black males m the South. Geo- graphical mortahty patterns observed were sinnlar to those of oesophagus and lung cancer. Conclusion: Tobacco smoking and alcohol drinking are the major risk factors for oropharyngeal cancer, as they are for oesophageal cancer while smoking is linked to lung cancer. These temporal and geographical trends should be essentxally interpreted m terms of patterns and changes m exposure of these two rtsk factors, suggesting further efforts to control them.