Abstract Assessment of moringa oleofera seed powder on the shelf life of smoked Clarias gariepinus. Fifteen table sized C. gariepinus with an average weight of 550g were used and were assigned into five treatments. The five treatments were soaked in M. oleifera marinade (MOM) at diferent inclusion level. These are: the control with 0% MOM, 10g MOM inclusion, 20g MOM inclusion, 30g MOM inclusion and 40g MOM inclusion in 1000cm3 of water. The fish were gutted, washed and randomly assigned to the treatments. Thereafter, the fishes were soaked in the treatments for one hour and later hot smoked. After smoking, the fishes were stored in netted boxes and placed on laboratory shelves for two months. Microbial counts and proximate composition were conducted on the various treatments at 7-day interval for four weeks. There was a general increase in microbial load as storage progressed. There was a general increase in microbial load as storage progressed. However, the increment was pronounced in the control fish samples. In all levels of MOM, there was decrease in the bacterial and mold/yeast counts as compared with the control samples. 4% MOM exhibited the highest antibac- terial potency and antifungal potency as well. Of all the nutrients which are Protein, fat, fibre, ash, moisture and carbohydrate, only protein and moisture increased generally as storage weeks progressed. The other nutrients decreased. M. oleifera marinade could be used to protect stored smoked catfish from microbial spoilage thus limiting economic loss and possible health risk to consumers. It was concluded that using M. oleifera seed as a preservative for smoked C. gariepinus enhances the nutrient quality and reduces the microbial loads. Therefore, preservation of feed with M. Oleifera seed should be encouraged. Volume 3 • Issue 1 • 2021 Copyright © All rights are reserved by Edward A. Journal of Agriculture and Aquaculture Research Article Edward A* Department of Fisheries and Aquaculture, Adamawa State University, Mubi- Nigeria Received: March 16, 2021; Published: August 19, 2021 *Corresponding Author: Edward A, Department of Fisheries and Aquaculture, Adamawa State University, Mubi-Nigeria. Assessment of Moringa Oleofera Seed Powder on the Shelf Life of Smoked Clarias Gariepinus Citation: Edward A (2021). Assessment of Moringa Oleofera Seed Powder on the Shelf Life of Smoked Clarias Gariepinus. Journal of Agri- culture and Aquaculture 3(1). Fish is an indispensable source of animal protein, essential fatty acids, minerals and vitamins. Its amino acids composition very well suited human dietary requirements, competing favorably with egg, milk and meat in its nutritional value (Feldhusen, 2000). Fish protein is relatively cheaper and richer in lysine and other Sulphur amino acids than other livestock protein thus suitable for comple- menting high carbohydrate diets (Abdullahi et al., 2001). Fish is highly perishable. It is readily susceptible to chemical and micro- bial deterioration leading to economic loss, reduction in quality attributes and wastage (Gram and Huss, 2001). Food wastage and spoilage has been recognized as a significant constraint in achiev- ing the much-desired self-sufficiency in food and fibre production in Africa (Food and Agriculture Organization, 2000). Presently, about two-third of the world population subsist on poorly balanced diets that retard normal growth and development (Food and Agriculture Organization, 2012). Shelf life is defined as the period of time a product is fit for consumption; it is a relatively short period for fresh fish stored under refrigerated conditions (12 Introduction