16 Hygeia.J.D.Med.10 (1) August 2018; 16-26 Hygeia.J.D.Med. August 2018 – January 2019 ISSN 2229 3590 Hygeia:: journal for drugs and medicines August 2018 Open Access www.hygeiajournal.com Research article section: Medicinal Natural Products A Half Yearly Scientific, International, Open Access Journal for Drugs and Medicines DOI:10.15254/H.J.D.Med.10.2018.174 Research Article IN VITRO ANTI-INFLAMMATORY AND CYTOTOXICITY STUDIES OF TWO MANGROVE ASSOCIATED LICHENS, Dirinaria consimilis AND Ramalina leiodea EXTRACTS VINAY BHARADWAJ TATIPAMULA* AND GIRIJA SASTRY VEDULA Pharmaceutical Chemistry Department, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam – 530 003, Andhra Pradesh, India. Keywords: Inflammation, cancer, protein denaturation method, SRB assay, cytotoxicity Correspondence: Vinay Bharadwaj Tatipamula M.Pharm, Ph.D Pharmaceutical Chemistry Department, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India. – 530 003 Received: 15 April 2018, Revised: 9 May 2018 Accepted: 28 May 2018, Available online: 15 September 2018 ABSTRACT Plan: To evaluate the anti-inflammatory and cytotoxicity activity of different extracts from two different manglicolous lichens (Dirinaria consimilis and Ramalina leiodea). Preface: Inflammation is the origin of several deadly diseases like cancer, atherosclerosis, Alzheimer’s and rheumatoid arthritis. From decades, lichen extracts and its metabolites are well known in treating inflammation and cancer. Outcome: The outcome of protein denaturation method confirmed that the ethyl acetate and acetone extract of R. leiodea depicted better inhibitory profile against protein denaturation with IC50 values of 268 and 330 μg/mL Furthermore, the results of SRB assay showed that ethyl acetate and acetone extracts of both the lichens acts potently against MCF-7, DLD-1, HeLa and A549. Simultaneously, all the tested extracts depicted low degree of specificity towards NHME, they are less toxic. Hence, further screening of these extracts may lead to the exploration of safe and potent anti-inflammatory and anticancer agents. 1. INTRODUCTION Inflammation is a response to any sort of physically contusion like injured cells, pathogens or irritants 1 . Inflammatory process which under usual conditions is self-limiting becomes continuous and chronic inflammatory syndromes flourish eventually 2 . The chronic inflammatory syndrome leading to dangerous diseases like appendicitis, bronchitis, dermatitis, meningitis, sinusitis, sore throat, tonsillitis, aging, Alzheimer’s, asthma, atherosclerosis, cancer, Crohn’s disease, hepatitis, peptic ulcer, periodontitis, psoriasis, rheumatoid arthritis, sclerosis, sepsis and tuberculosis diseases 3 . Corresponding author email: vinaybharadwajt@gmail.com, Mobile: +91-9640325675 Hygeia.J.D.Med. Vol.10 (1), August 2018 © All rights reserved Hygeia journal for drugs and medicines, 2229 3590 ORCID ID: 0000-0002-9376-1911, Researcher id: L-8352-2018