Medical Laboratory Technology Journal Copyright © 2019, MLTJ, ISSN 2461-0879 Corresponding Author: Meilla Dwi Andrestian Department of Community Nutrition, Faculty of Human Ecology, IPB University, Kampus IPB Dramaga Bogor, West Java, Indonesia 16680 Email: meilladwi74@gmail.com Anti-hyperglycemic Effect of Torbangun (Coleus amboinicus Lour) Leaves Extract Through Liver and Muscle Glycogen Deposits in Streptozotocin-induced Hyperglycmic Sprague-Dawley Rats Model *Meilla Dwi Andrestian 1,2 , Rizal Damanik 2,3 , Faisal Anwar 2 , Nancy Dewi Yuliana 4 1 Departement of Nutrition, Banjarmasin Health Polytechnic, Banjarbaru, South Kalimantan, Indonesia 70714, 2 Department of Community Nutrition, Faculty of Human Ecology, IPB University, Bogor, West Java Indonesia 16680, 3 Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center IPB University, Bogor, West Java, Indonesia 16680, 4 Department of Food Science, Faculty of Agricultural Engineering and Technology IPB University, Bogor, West Java, Indonesia 16680. Email: meilladwi74@gmail.com DOI: 10.31964/mltj.v%vi%i.227 Abstract: The association of liver and muscle glycogen deposits with serum insulin levels, β-cells pancreas, and fasting blood glucose (FBG) of streptozotocin (STZ)- induced hyperglycemic rats receiving Torbangun leaves extract (TE) investigated. The intervention performed on 25 8-week-old Sprague-Dawley rats divided into four groups. Seven rats separated as a normal group (N), and other rats injected with streptozotocin (STZ). Confirmation of hyperglycemic was characterized by fasting blood glucose >126 mg/dl. Treatment group which is NG (hyperglycemic rats); N (normal rats); H-IM (62.5 mg/kg BW metformin); and H-IT (620 mg/kg BW TE) for 14 days. This study revealed that TE significantly decreased FBG levels, increased insulin production, and the amount of liver glycogen deposits (a=0.01). However, the intervention did not significantly increase the amount of muscle glycogen deposits. TE administration improves β-cells, increases the liver and muscle glycogen deposits. TE was shown to have antihyperglycemic activity by improving the β-cell, increasing blood serum insulin levels, decreasing blood glucose levels, and increasing the liver glycogen deposits. Keywords: diabetes; glycogen; hyperglycemia; torbangun (Coleus amboinicus Lour) INTRODUCTION Glycogen, which is the main carbohydrate deposit in the body, is mainly localized in the liver and skeletal muscle. It acts as a source of glucose when the body requires glycogen breakdown for energy metabolism, which referred to as glycogenolysis (Gropper and Smith, 2013). The liver and muscle glycogen deposits of people with diabetes decrease because the glucagon activity increases with decreased insulin production, which is responsible for stimulating glycogen formation. Individuals with type 1 diabetes mellitus (DM) cannot adequately produce insulin. Meanwhile, individuals with type 2 DM can produce insulin in a fixed amount, but the insulin does not function properly (insulin resistance) because the insulin receptors in the cell membrane reduced or their structures altered. Thus, the receptors are unable to respond to the presence of insulin. This condition results in hyperglycemia because glucose cannot be stored in the form of glycogen, either in the liver or muscles. Medical Laboratory Technology Journal Available online at : http://ejurnal-analiskesehatan.web.id Received 2019-14-08; Revised 2019-21-08; Accepted 2019-22-10