http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, Early Online: 1–2 ! 2015 Informa UK Ltd. DOI: 10.3109/19401736.2014.1003874 MITOGENOME ANNOUNCEMENT The complete mitochondrial genome of the Vermiculated angelfish (Chaetodontoplus mesoleucus) (Perciformes: Pomacanthidae) Chung-Der Hsiao 1 , Kang-Ning Shen 2 , Ching-Hung Chen 3,4 , and Chih-Wei Chang 5,6 1 Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, Taiwan, ROC, 2 Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, ROC, 3 WeThink Biotech INC., Taoyuan, Taiwan, ROC, 4 Zgenebio Biotech Inc., Taipei, Taiwan, ROC, 5 National Museum of Marine Biology and Aquarium, Checheng, Pingtung, Taiwan, ROC, and 6 Graduate Institute of Marine Biodiversity and Evolutionary Biology, Checheng, Pingtung, Taiwan, ROC Abstract In this study, the complete mitogenome sequence of the Vermiculated angelfish (Chaetodontoplus mesoleucus) (Perciformes: Pomacanthidae) has been sequenced by the next-generation sequencing method. The assembled mitogenome consisting of 16,998 bp, including 13 protein coding genes, 22 transfer RNAs, and two ribosomal RNAs genes. The overall base composition of vermiculated angelfish is 27.6% for A, 30.7% for C, 16.1% for G, and 25.6% for T and show 85% identities to Bluestripe angelfish C. septentrionalis in the same genus. The complete mitogenome of the Vermiculated angelfish provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for marine angelfishes. Keywords Mitogenome, next-generation sequencing, Vermiculated angelfish History Received 2 December 2014 Accepted 5 December 2014 Published online 29 January 2015 The Vermiculated angelfish Chaetodontoplus mesoleucus (Bloch, 1787), also known as the Singapore angelfish, has been likened to a butterflyfish in appearance. It has a blue lips and a yellow nose abruptly change to a black vertical band over the eyes, resembling a mask. The rest of the body gradually changes from white to dark grey posteriorly with a caudal fin in bright yellow. It occurs in tropical Indo-West Pacific, from South-east Asia, north to Japan, south to Australia, and east to the Solomon Islands. The complete mitogenome of the Vermiculated angelfish can provide essential and important DNA molecular data for further phylogeography and evolutionary analysis for marine angelfishes. Samples of C. mesoleucus were collected from Cebu, Philippines. The methods for genomic DNA extraction, library construction, and next-generation sequencing were followed our previous publication (Shen et al., 2014). We performed de novo assembly by using commercial software (Geneious V7, Auckland, New Zealand) to produce a single, circular form of complete mitogenome with about an average 65 coverage (3396 out of 7,483,038 reads, 0.045%). The 1197 bp highly variable D-loop region was validated by Sanger sequencing with two primers of D-loop seqF (5 0 -AGAGCGCCGGTCTTGTAA-3 0 ) and D-loop seqR (5 0 -GCGGATACTTGCATGTGTAAG-3 0 ). The complete mitochondrial genome of C. mesoleucus was 16,998 bp in size (GenBank: KP218262) and its overall base composition is 27.6% for A, 30.7% for C, 16.1% for G, and 25.6% for T and show 85% identities to Bluestripe Angelfish C. septentrionalis in the same genus. The complete mitogenome of C. mesoleucus includes 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes (Table 1). The protein coding and tRNA genes of C. mesoleucus mitogenome were predicted by using DOGMA (Wyman et al., 2004), ARWEN (Laslett & Canback, 2008), and MitoAnnotator (Iwasaki et al., 2013) tools. All protein-coding genes were encoded on the H-strand with exception of ND6. Most tRNA genes were encoded on the H-strand with exception of tRNA-Gln, tRNA-Ala, tRNA-Asn, tRNA-Cys, tRNA-Tyr, tRNA-Ser, tRNA-Glu, and tRNA-Pro. All the 13 mitochondrial protein-coding genes share the start codon ATG, except for ND2 (CTC) and CO1 (GTG). It also important to note that only one of the 13 protein- coding genes is inferred to terminate with TAA termination codon (ATP8), while others are terminated with codons of ATA (ND1), ACT (ND2), TCT (CO1), TCA (CO2), GTA (ATP6), TCA (CO3), GAA (ND3), TGC (ND4L), GCC (ND4), TTC (ND5), GTT (ND6), and TTA (CYTB). The longest one is ND5 gene (1839 bp) in all protein coding genes, whereas the shortest is ATP8 gene (168 bp). The two ribosomal RNA genes, 12S rRNA gene (954 bp) and 16S rRNA gene (1804 bp), are located between tRNA-Phe and tRNA-Leu (UAA) and separated by tRNA-Val. We expect that the present result would elucidate the further phylogenetic approach among different species of marine angelfish. Correspondence: Chih-Wei Chang, National Museum of Marine Biology and Aquarium, Checheng, Pingtung 944, Taiwan, ROC. E-mail: changcw@nmmba.gov.tw Mitochondrial DNA Downloaded from informahealthcare.com by National Taiwan University on 01/30/15 For personal use only.