Doi: 10.21059/buletinpeternak.v42i2.13609 Supplementation of Follicle Stimulating Hormon Into In vitro Maturation Medium to Increase Oocytes Maturation and 4 Cell Stadium Embryo Development of Bligon Goat Yanuar Achadri *1 , Diah Tri Widayati 2 , and Sigit Bintara 2 1 Physiology and Reproduction of Animal Science, Universitas Islam Batik, Surakarta, Jawa Tengah, 57147, Indonesia 2 Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia Article history Submitted: 6 October 2016 Accepted: 27 April 2018 * Corresponding author: Telp. +62 81233272406 E-mail: yanuar.achadri13@mail.ugm.ac.id ABSTRACT The study was carried out to investigate the effect of follicle stimulating hormon (FSH) into in vitro maturation medium to increase oocytes maturation and 4 cell stadium embryo development of Bligon goat. Goat ovaries were obtained from a slaughterhouse and transported to the laboratory in a flask of NaCl at temperature of 31 – 34°C. Oocytes were aspirated from 2 – 6 mm of follicles into a 3 mL syringe (23G needle) that contained Dulbecco’s Phosphate-Buffered Saline. Oocytes were divided into three groups, i.e tissue culture medium (TCM) with FSH supplementation 0, 50, and 100 IU/mL. Oocytes were put into those medium and incubated on 39°C, 5% CO 2 , and 95% humidity for 24 hours. Matured oocytes were fertilized with capacitated frozen thawed-semen and incubated on 39°C, 5% CO 2 , and 95% humidity for 5 hours. Fertilized oocytes were washed for 3 times in TCM and incubated in the same condition for embryo culture. The data of FSH supplementation and embryo development were analyzed using randomized completely one way classification. The results showed that the percentages of mature oocytes from FSH supplementation 0, 50, and 100 IU/mL were 70,48±23,22, 78,48±15,80, and 80,29±12,86%, respectively. Cleavage rate of the two cells stage were 36,00±14,22, 44,00±33,94, and 57,45±31,78%, respectively, and for the 4 cells stage were 27,33±22,04, 35,33±40,73, and 39,45±20,38%. It is concluded that supplementation of FSH in the maturation medium could not increase the percentages of in vitro maturation and embryo development. Keywords: Bligon goat, Oocytes, Follicle stimulating hormon, In vitro embryo production Introduction Goat play an important role as a source of revenue and spur a national program to increase per capita consumption of animal protein. The rate of goat population growth in the last five years relatively low, less than 3.5 percent per year. Bligon goat is a crossbreed between Ettawa goat with Kacang goat (Nuraini et al., 2014). Ovary as the by-products of goats from the slaughter process can be used for reproduction technology. One of the recent technologies that can increase the population and productivity of Bligon goat is the application of in vitro fertilization reproduction technologies (Herdis, 2014). In vitro fertilization process involves the oocytes recovery from ovary follicles, oocytes in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro culture of the fertilized oocytes until it become an embryo (Boediono et al., 2000). One of the main factor to improve an in vitro embryo production is by the hormone supplementation on the maturation medium. Reproductive hormones play an important role in livestock reproduction activities. One of the reproductive hormones is Follicle Stimulating Hormone (FSH). Follicle stimulating hormone is used as an essential component in culture medium, culture processes, and for maintaining viability and oocyte growth. Follicle stimulating hormone with growth factor can stimulate cumulus cells to produce and secrete a hyaluronic acid that will disperse the cell, this process is called expansion (Ciptadi et al., 2011). Follicle stimulating hormone in maturation medium will stimulate and regulate chromatin condensation for meiotic division, besides FSH will stimulate cAMP and cumulus cell expansion (Wattimena, 2011). Optimal expansion of the cumulus cells appears to be essential for the successful of in vitro maturation, influence the process of in vitro fertilization and in vitro embryo development (Zhou and Zhang, 2005). Buletin Peternakan 42 (2): 90-96, May 2018 Bulletin of Animal Science ISSN-0126-4400/E-ISSN-2407-876X Acredited: 36a/E/KPT/2016 http://buletinpeternakan.fapet.ugm.ac.id/ 90