Research Article The CYBA Gene ∗ 49A>G Polymorphism (rs7195830) Is Associated with Hypertension in Patients with Coronary Artery Disease Tomasz Nowak, 1 PaweB Niemiec, 1 Sylwia GórczyNska-Kosiorz, 2 Anna Balcerzyk, 1 Tomasz Iwanicki, 1 Jolanta Krauze, 3 Wladyslaw Grzeszczak, 2 Anna Ochalska-Tyka, 4 Joanna Iwanicka, 1 and Iwona Zak 1 1 School of Health Sciences in Katowice, Department of Biochemistry and Medical Genetics, Medical University of Silesia, Medyk´ ow Street 18, 40-752 Katowice, Poland 2 School of Medicine and Division of Dentistry in Zabrze, Department of Internal Medicine, Diabetes and Nephrology, Medical University of Silesia, 3 Maja Street 13-18, 41-800 Zabrze, Poland 3 1st Department of Cardiac Surgery/2nd Department of Cardiology, American Heart of Poland, S. A. Armii Krajowej Street 101, 43-316 Bielsko-Biala, Poland 4 Regional Centre of Blood Donation and Blood Treatment in Raciborz, Sienkiewicza Street 3, 47-400 Raciborz, Poland Correspondence should be addressed to Tomasz Nowak; tnowak@sum.edu.pl Received 2 February 2016; Accepted 4 May 2016 Academic Editor: Sanjay K. Banerjee Copyright © 2016 Tomasz Nowak et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose. Single nucleotide polymorphisms of the CYBA gene may modify the risk of coronary artery disease (CAD). Te aim of the present study was to investigate whether the ∗ 49A>G (rs7195830) polymorphism is associated with CAD. Materials and Methods. CYBA gene ∗ 49A>G polymorphism was determined in 481 subjects: 242 patients with premature CAD and 239 age and sex matched controls using the fuorescently labeled allele-specifc oligonucleotides method. Results. Te frequency of the ∗ 49G allele carrier state was signifcantly higher in patients than in controls (84.8% versus 76.6%, resp.,  = 0.020), as well as the frequency of the ∗ 49G allele (62.2% versus 54.0%,  = 0.009). Both factors were associated with CAD in the analyzed population (OR = 1.70, 95% CI: 1.04– 2.76 for GG+AG versus AA and OR = 1.40, 95% CI: 1.08–1.83 for ∗ 49G versus ∗ 49A). Carrier state of the ∗ 49G allele was a stronger and independent risk factor for CAD among women (OR = 4.35, 95% CI: 1.50–13.20,  = 0.002), as well as the ∗ 49G allele (OR = 2.25, 95% CI: 1.34–3.77,  = 0.001). Te ∗ 49G allele carrier state was also associated with lef ventricular hypertrophy in patients with coronary artery disease ( = 0.015). Conclusion. Te CYBA gene ∗ 49A>G polymorphism modifes the risk of coronary artery disease. 1. Introduction NADPH oxidases (nicotinamide adenine dinucleotide phos- phate oxidases) are one of the main sources of reactive oxygen species (ROS) in human body and especially in vasculature. NADPH oxidases are expressed in endothelium, vascular smooth muscle cells, fbroblasts, cardiomyocytes, and mainly phagocytes. Free radicals produced by NADPH oxidases are involved in the regulation of ROS-dependent intracellular signaling pathways, afecting expression of many classes of genes. In physiological conditions, ROS can stimulate growth, diferentiation, migration, division, and apoptosis of cells of the vessel wall, to regulate vascular remodeling processes [1– 5]. When synthesized in excessive levels, ROS may intensify the progress and development of atherosclerosis. Many data suggest the role of NADPH oxidases in the pathogenesis of cardiovascular disorders with atherosclerotic background, including coronary artery disease (CAD) [3, 6–9]. NADPH oxidases are multisubunit enzymatic complexes, which produce superoxide anion from molecular oxygen [6, 10]. Teir active complexes are composed of NOX (NADPH oxidase) catalytic protein and many auxiliary subunits. Tere Hindawi Publishing Corporation BioMed Research International Volume 2016, Article ID 1539671, 7 pages http://dx.doi.org/10.1155/2016/1539671