Physiological
Communication
Biosci. Biotech. Res. Comm. 9(3): 341-348 (2016)
Cognitive type susceptibility in ICR mice model of
chronic cerebral hypoperfusion.
Sirilak Somredngan
1
and Wachiryah Thong-asa
1
*
1
Physiology Division, Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
ABSTRACT
Animal model of chronic cerebral hypoperfusion (CCH) was found to be benefcial as pathophysiological provided
relevance to vascular dementia (VD) and subcortical ischemic vascular dementia (SIVD). There were many evidence
kinds of CCH animal model and all were useful. The present study investigated the pathophysiology of mild CCH in
ICR mice during 2 to 8 weeks of permanent right common carotid artery occlusion Sensorimotor, cognitive abilities
and anxiety-like behavior were assessed in Morris water maze (MWM) and elevated plus maze (EPM), respectively.
Frontal cortex, striatum and hippocampus infarctions were evaluated using 2% 2,3,5-triphenyltetrazolium chloride
(TTC) at 2, 4 and 8 weeks of permanent right common carotid arteryocclusion. Sensorimotor, spatial learning and
memory in the acquisition paradigm of the MWM and anxiety-like behavior assessed in the EPM were not affected
during the experimental period of 2, 4 and 8 weeks of arterial occlusion.Signifcant defcit of learning fexibility and
memory of reverse platform location in the reversal paradigm were found, though reversible of learning fexibility
defcit presented at 8 weeks. Signifcant infarction early appeared in striatum since 4 weeks while the frontal cortex
and hippocampus stated at 8 weeks. The present study suggested that mild CCH induced by permanent right com-
mon carotid artery occlusion in ICR mice induced reversible learning fexibility defcit but not memory of the reverse
platform location in the MWM.
KEY WORDS: CHRONIC CEREBRAL HYPOPERFUSION, ELEVATED PLUS MAZE,LEARNING FLEXIBILITY, SPATIAL LEARNING AND MEMORY.
341
ARTICLE INFORMATION:
*Corresponding Author: fsciwyth@ku.ac.th
Received 20
th
Aug, 2016
Accepted after revision 7
th
Sep, 2016
BBRC Print ISSN: 0974-6455
Online ISSN: 2321-4007
Thomson Reuters ISI ESC and Crossref Indexed Journal
NAAS Journal Score 2015: 3.48 Cosmos IF : 4.006
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Online Contents Available at: http//www.bbrc.in/
INTRODUCTION
Animal model of chronic cerebral hypoperfusion (CCH)
can be induced by permanent ligation of major cerebral
arterial supplies. Severity of pathological appearances
depended on the number of vessel occlusion and persis-
tent of cerebral reduction. CCH study in animal model
is associated with cerebral blood fow (CBF) reduction,
metabolic insuffcient, oxidative stress, neuroinfam-
mation, neurotransmitter system dysfunction, mental
confusion, cognitive decline, white matter and neu-
ronal degeneration. These pathological relevancies of