40 Isnaeni dkk, Pengaruh Pemberian Omega-3, Pendahuluan Burung puyuh dapat ditemukan di hampir seluruh bagian dunia, hidupnya kebanyakan masih liar dan hanya sebagian kecil yang sudah dibudi dayakan (Nugroho & Mayun 1981). Untuk budi daya puyuh perlu dilakukan breeding. Untuk breeding dibutuhkan berbagai hal, antara lain ketersediaan bahan pakan dengan khasiat yang tepat serta bibit puyuh jantan yang berkualitas. Persoalannya, bibit burung puyuh jantan yang dibutuhkan untuk keperluan breeding tersebut (khususya puyuh Pengaruh Pemberian Omega-3, Omega-6, dan Kolesterol Sintetis terhadap Kualitas Reproduksi Burung Puyuh Jantan (Omega-3, Omega-6 and Synthetic Cholesterol Effect towards Male Quail Reproduction Quality) Wiwi Isnaeni 1] , Abyadul Fitriyah 2] , dan Ning Setiati 1] 1] Jurusan Biologi Fakultas MIPA Universitas Negeri Semarang Jalan Raya Sekaran Gunungpati Semarang 50229 e-mail: wi2isna@yahoo.co.id 2] Jurusan Reproduksi Ternak Fakultas Peternakan Universitas Nahdlatul Wathan Mataram Jalan Kaktus 1-3 Mataram, Lombok e-mail: !try_amin@yahoo.com Abstract The aims of this research were to study the effect of using the omega-3 (O-3), omega-6 (O-6), and synthetic cholesterol (Ch) on the reproductive quality of the male quails. Samples of this research were 35 of 4 weeks old male quails with 60 to 98 g of weight. The samples were grouped randomly into 7 groups, that are R-0 (control-group), R-1 (0,163 mg O-3/100 g body weight), R-2 (0,326 mg O-3/100 g bw), R-3 (0,163 mg O-6/100 gbw), R-4 (0,326 mg O6/100 g bw), R-5 (20 mg Ch/100 g bw), and R-6 (40 mg Ch/100 g bw). The treatment was given per oral in three periods with 5 days of each. there was a week intervening between periods. During the research, samples were gifted rations a basalic-woof (basalic-feed) for twice/day, at 7:00 and 16:00 oclock. the amount of basalic-woof were 20 g of feed/100 g body weight/day. Water administered in an ad libitum manner. This research was implemented during 8 weeks. The variables were the level of testosterone, blood qualities, voice-qualities, color brightness of chest fur, and testis size. The voice quality data were analyzed by one-way ANOVA continued by orthogonal contrast test. The other data were analyzed by descriptive statistic. The result of this research con!rmed that synthetic omega-3 and omega-6 (0,326 mg/100 g bw) and synthetic cholesterol (20 mg/100 g bw) could increase the reproductive quality of the male quails. Key words : synthetic cholesterol, synthetic omega-3, synthetic omega-6, reproductive quality