Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Tue, 02 Apr 2019 22:05:15 Serinibacter salmoneus gen. nov., sp. nov., an actinobacterium isolated from the intestinal tract of a fish, and emended descriptions of the families Beutenbergiaceae and Bogoriellaceae Moriyuki Hamada, Takao Iino, Tomohiko Tamura, Takahiro Iwami, Shigeaki Harayama and Ken-ichiro Suzuki Correspondence Moriyuki Hamada hamada-moriyuki@nite.go.jp NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan A novel Gram-positive bacterium, designated Kis4-28 T , was isolated from the intestinal tract of a fish, and its taxonomic position was investigated by a polyphasic approach. The sample was collected from the coast of Tokyo Bay, Japan. Cells of strain Kis4-28 T were rod-shaped, non- motile and non-sporulating. The peptidoglycan type of the isolate was A4a; lysine was the diagnostic diamino acid. The only menaquinone detected was MK-8(H 4 ), and the major fatty acids were anteiso-C 15 : 0 and C 16 : 0 . Galactose was detected as a major cell-wall sugar. The polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 70.7 mol%. 16S rRNA gene sequence analysis revealed that strain Kis4-28 T and the type strain of Salana multivorans formed a monophyletic cluster with a 16S rRNA gene sequence similarity of 96.2 %. Strain Kis4-28 T was clearly distinguishable from the genus Salana in terms of its chemotaxonomic characteristics. On the basis of the genotypic and phenotypic characteristics, a new genus and species is proposed for strain Kis4-28 T , with the name Serinibacter salmoneus gen. nov., sp. nov. The type strain of Serinibacter salmoneus is Kis4-28 T (5NBRC 104924 T 5DSM 21801 T ). In addition, on the basis of 16S rRNA gene sequence analysis of the genus Serinibacter and related genera, emended descriptions of the families Beutenbergiaceae and Bogoriellaceae are proposed to accommodate the genera Beutenbergia, Salana and Serinibacter, and the genera Bogoriella and Georgenia, respectively. The suborder Micrococcineae was proposed by Stackebrandt et al. (1997). So far, 15 families have been assigned to this suborder (Stackebrandt et al., 1997; Stackebrandt & Schumann, 2000; Li et al., 2005, 2008; Zhi et al., 2009). Of them, the genera Beutenbergia (Groth et al., 1999), Salana (von Wintzingerode et al., 2001) and Georgenia (Altenburger et al., 2002) have been proposed as the members of the family Beutenbergiaceae (Zhi et al., 2009). It has been reported that the genus Ruania is phylogenetically related to the genera Georgenia, Bogoriella (Groth et al., 1997), Beutenbergia and Salana, but the family to which the genus Ruania belongs has not been specified (Gu et al., 2007). Members of the suborder Micrococcineae are widespread and occur in both natural and artificial habitats, e.g. in soil, plants, sediments, food and dairy products. While isolating bacteria from marine samples, we isolated a novel actinobacterium (strain Kis4-28 T ) from the intestinal tract of a fish species collected from Tokyo Bay, Japan. Comparative 16S rRNA gene sequence analysis revealed that strain Kis4-28 T is a member of the suborder Micrococcineae. The objective of this study was to determine the taxonomic position of this strain by using a polyphasic approach. Japanese sillago (Sillago japonica), a type of fish, was collected from Kyonan beach on the coast of Tokyo Bay, Japan. The sillago was dissected, and an intestinal tract sample of approximately 1 g was used for the isolation of bacteria. The procedure employed for bacterial isolation was as described by Iino et al. (2007). NBRC medium 802 containing 1.0 % Polypepton (Wako), 0.2 % yeast extract (Difco), 0.1 % MgSO 4 . 7H 2 O and 1.5 % agar (if required) was used for general laboratory cultivation, morphological studies and for determining the optimal growth para- The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Kis4-28 T is AB455532. A phylogenetic tree derived from 16S rRNA gene sequences of strain Kis4-28 T and its taxonomic neighbours, including many strains in the suborder Micrococcinae, is available as a supplementary figure with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2009), 59, 2809–2814 DOI 10.1099/ijs.0.011106-0 011106 G 2009 IUMS Printed in Great Britain 2809