D-dimer plasma level in ALL patient and relationship with thrombosis, DIC (Disseminated Intravascular Coagulation) and thromboambolic accident. Method: In this study 30 cases of ALL admitted in Ahwaz Shafa hospital hematology–oncology center were participated. Samples from 30 age and sex matched normal controls were also collected for coagulation analysis. Plasma level of D-dimer was measured with enzyme-linked immunosorbent assay (ELISA) (Diagnostica Stago) and statistic analysis was done using one way ANOVA and sample T-Test. Results: In this result mean level of D-dimer in patient elevated statistically significant compared with control group (P=0.0001). Conclusion: Regarding thrombocytopenia and elevated of D-dimer level thrombosis risk, DIC and thromboambolic complication increased in ALL patients, thus for decreasing these symptoms and increasing good prognosis preserving and preventing therapies with anticoagulant drugs are recommended. Keywords: Acute lymphoblastic leukemia, Thrombosis, DIC doi:10.1016/j.clinbiochem.2011.08.471 Poster—[A-10-356-1] Novel chlorotoxin-like peptide: Structure-function relationship study for therapeutic use on human glioma Rjeibi Ilhem 13, place of Pasteur Tunisia 1002 E-mail address: rjeibii@yahoo.fr Introduction: The prognosis for glioblastoma patients is poor despite treatment consisting of surgical debulking, radiotherapy, and chemotherapy. One type of targeted therapy which looks promising is based on the scorpion venom. The purpose of this study was to identify, characterize and synthesize the first chlorotoxin-like, named AaCtx, peptide from Androctonus australis that inhibit migration and invasion of glioma cells. Methods: After purification and characterization AaCtx was chemically synthesized by means of solid-phase technique. The experimental mass of sAaCtx (3590.126 Da) was obtained by MALDI ionization. Cell invasion assays were carried out using 24-well Transwell inserts with Millipore coated with a thin layer of Matrigel. In vitro cell migration assays were performed using modified Boyden chambers. Results: AaCtx is the first chlorotoxin-like peptide isolated from Androctonus australis scorpion venom. Its amino acid sequence shares 70% similarity with chlorotoxin from Leiurus quinquestriatus scorpion venom, from which it differs by twelve amino acids. AaCtx was chemically synthesized. Both native and synthetic AaCtx were active on invasion and migration of human glioma cells. However, their activity was found to be lower than that of chlorotoxin. The molecular model of AaCtx shows that most of amino acids differing between AaCtx and chlorotoxin are localized on the N-terminal loop and the α-helix. Conclusion: The data presented herein indicate that AaCtx represents a new member of the small family of peptides active on glioma cells. This finding may serve as a starting point for structure– function relationship studies leading to design high specific anti- glioma drugs. Keywords: Chlorotoxin-like peptide, Human glioma, Scorpion venom, Androctonus doi:10.1016/j.clinbiochem.2011.08.472 Poster – [A-10-365-1] Immunohistochemical study of expression of Glucose transporter 1 (GLUT1) aquaporin 1 (AQP1), Na, K-ATPase and Derlin-1 in canine normal, benign and malignant neoplastic mammary gland Golshahhi Hannaneh a , Ghasemi Elmira a , Mohamadi Fatemeh b , Shams Farhad b , Azadkar Yasaman c a Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran b Faculty of Medicine, Gilan University of Medical Sciences, Gilan, Iran c Faculty of Basic Science, University of Gilan, Gilan, Iran E-mail address: golshahi.vet@gmail.com (G. Hannaneh) Introduction: The altered growth of neoplastic cells necessitates alterations in cellular metabolism which, in turn, influences the cell surface expression of membrane proteins involved in water, nutrient and electrolyte homeostasis. Aquaporin 1 (AQP1), is a water channel that controls the permeability of endothelial and epithelial barriers by fasciliting water movement across cell membranes. the hypoxia- responsive glucose transporter( GLUT1) and Na, K-ATPase, an electro- genic transmembrane P-type ATPase responsible for establishing and maintaining the high intracellular potassium (150 mEql -1 K+) to low intracellular sodium (15 mEql -1 Na+) ratio characteristic of all living cells. Derlin-1 (Derl-1) is a transporter protein for the export of misfolded proteins from the endoplasmic reticulum (ER) and an inhibitor of ER stress-induced apoptosis. Material and methods: Fifteen samples of mammary tissue were collected for histopathological examination. Standard 4 μm thick sections were stained using haematoxylin and eosin (H and E) and using immunohistochemical techniques. Results: AQP1 immunoreactivity was absent from the majority of specimens studied. GLUT1 immunoreactivity was observed in normal mammary tissue and particularly in the epithelial and mesenchymal cells of benign, and in the epithelial cells of malignant tumours, respectively. Na, K-ATPase immunoreactivity was present in normal and neoplastic mammary epithelium and was significantly increased in the epithelium of both benign and malignant tumours. Weak Derl-1 expression was found in normal mammary tissue and benign tumour and moderate to marked expression in malignant tumours was seen. Conclusion: These results suggest that GLUT1 is more highly expressed in neoplastic epithelium and mesenchyme and that Na, K- ATPase is more highly expressed in neoplastic mammary epithelium. And also neoplastic mammary gland cells appear to have an increased stress-associated unfolded protein response. Thus the ubiquitous expression of Derl-1 in most cell types hinders its use as a marker of metastatic potential In consequence, these proteins may have potential as diagnostic and prognostic biomarkers of canine mammary neoplasia. Keywords: Dog, Immunohistochemistry, Mammary gland. doi:10.1016/j.clinbiochem.2011.08.473 Poster—[A-10-375-2] Study of the DNMT3B variant genotype among Iranian sporadic breast cancer in compare with normal individuals Rasti Mozhgan, Eftekhar Ebrahim, Sadeghi Yasaman, Jallali Mashayekhi Farideh, Fakhraddin Naghibalhossaini Shiraz, Iran E-mail addresses: rasti31@yahoo.com (R. Mozhgan), Eftekhar_msc@yahoo.com (E. Ebrahim), yasaman.zeus@yahoo.com (S. Yasaman), mashayekhif@yahoo.com (J.M. Farideh) Introduction: DNA methylation is a major epigenetic modification in humans, and aberrant DNA cytosine methylation may play an important role in carcinogenesis. The methylation of mammalian genomic DNA is Abstracts S194