INTRODUCTION
Trichoderma is a cosmopolitan fungus with a
worldwide distribution, common in soil, litter and
humus. It has cellulose decomposing ability of
municipal garbage (Samuels, 1996), and effective as
biocontrol agents against soil born plant pathogens
(Kelley, 1976; Jeffers and Wilcox, 1990). It is
reported that T. harzianum is capable of degrading
organochlorine pesticides such as DDT, dieldrin,
endosulfan, pentachloronitrobenzene, pentachlorophenol
and hence has potential applications for
bioremediation (Kelley, 1976).
Solid wastes, which contribute to air, water and land
pollution, can be classified into domestic, industrial
and agricultural (Drobny et al ., 1971; Bond and
Straub, 1973; Sell, 1981). Everyday huge amount of
solid wastes are generated in all the developed and
developing countries. Although developing countries
spend 20 to 40% of Municipal reserves for waste
management, but a significant amount of solid waste in
urban centers remain untreated (Thomas-Hope,
1998; Bartone, 2000 and Schubeler, 2000). Only a few
Asian cities like Tokyo, Singapore and Hong Kong
have adequate solid waste disposal facilities.
Bangladesh also generates huge amount (0.5 kg/capita/
day) of solid waste which creating environmental
hazards and have adverse effect on health (Zurbrügg,
2002). To overcome the ever-increasing serious
problem, conversion of solid waste into organic
compost seems to be a fruitful solution (Walker and
Willson, 1973 and Wootton et al ., 1981).
Therefore, the present study was undertaken to screen
the potential strains of Trichoderma for their efficiency
as effective bioconversion agents on solid waste.
MARETIALS AND METHODS
For effective decomposition of organic solid waste,
five local Trichoderma isolates, i.e. T. virens (IMI-
392430), T. pseudokoningii (IMI-1392431), T.
harzianum (IMI-392432), T. harzianum (IMI-
392433) and T. harzianum (IMI-392434) were
collected from stock culture of Biotechnology and
Plant Environ. Dev. 1(1):61-66, 2007 (June)
©Department of Botany, University of Rajshahi,
e-mail: plant.environ.dev@gmail.com
ISSN 1994-1501
Mode of action of Trichoderma spp. on organic solid waste for bioconversion
M.A. Bari, M.F. Begum, K.K. Sarker, M.A. Rahman, A.H. Kabir and M.F. Alam*
Department of Botany
University of Rajshahi, Rajshahi-6205, Bangladesh.
*e mail: falambt@yahoo.com
Abstract
Five strains of Trichoderma were used to select the most effective strain for bioconversion of Municipal solid
waste. Culture pellet was found more effective than spore suspension. The highest weight loss was 31.80 %,
when culture pellet (6 pieces) was used and it was 12% higher than control. Autoclaved solid waste was good
for degradation than non-autoclaved. Among the studied strains, T. harzianum (IMI-392432) was found most
effective. Using combination of Trichoderma showed 10% better result than single strain used.
Keywords: Bioconversion, Organic solid waste, Trichoderma , Culture pellet, Spore suspension.