1 Aging-associated Renal Disease in Mice is Fructokinase Dependent 1 2 Carlos A. Roncal-Jimenez 1 *, Takuji Ishimoto 1,2 *, Miguel A Lanaspa 1 , Tamara Milagres 1 , Ana 3 Andres Hernando 1 , Thomas Jensen 1 , Makoto Miyazaki 1 , Tomohito Doke 2 , Takahiro Hayasaki 2 , 4 Takahiko Nakagawa 3 , Shoichi Marumaya 2 , David A. Long 4 , Masanari Kuwabara 1 , Laura G. 5 Sánchez-Lozada 5 , Duk-Hee Kang 6 , Richard J Johnson 1,7 6 *equal contribution 7 From the 1 Division of Renal Diseases and Hypertension, University of Colorado, Aurora, 8 Colorado; 2 Department of Nephrology, Nagoya University Graduate School of Medicine, 9 Nagoya, Japan; 3 TMK Project, Medical Innovation Center, Kyoto University, Kyoto, Japan; 10 4 Developmental Biology and Cancer Programme, UCL Institute of Child Health, London, 11 UK; 5 Laboratory of Renal Physiopathology and Department of Nephrology, Instituto Nacional de 12 Cardiologia I.Ch., Mexico City, Mexico; 6 Department of Internal Medicine, Ewha Womans 13 University School of Medicine, Ewha Medical Research Center, Seoul, Republic of Korea; 14 7 Division of Nephrology, Eastern Colorado Health Care System, Department of Veteran Affairs, 15 Denver, Colorado; 16 17 Correspondence: Carlos Roncal-Jimenez, Division of Renal Diseases and Hypertension, 18 University of Colorado, Aurora, Colorado, email carlos.roncal@ucdenver.edu, phone 303-724- 19 4852 20 21 Running Title: Fructose Metabolism and Aging 22 Supported by Veteran’s Health Merit Grant from the National Institutes of Health 23 (BXI01BX002586-01) 24 25 Text: Abstract 222 words; Text 3032 words; Figures 7; Tables 1; References 36 26