Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Wed, 05 Dec 2018 12:16:06 Planobispora siamensis sp. nov., isolated from soil Wipaporn Ngaemthao, 1 Chanwit Suriyachadkun, 1 Suwanee Chunhametha, 1 Tomohiko Tamura 2 and Jean-Jacques Sanglier 1,3 Correspondence Chanwit Suriyachadkun chanwit@biotec.or.th 1 BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand 2 Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0812, Japan 3 Novartis Institutes for BioMedical Research Basel, Natural Products Unit, Novartis Pharma AG, Basel, Switzerland A novel actinomycete strain, A-T 4600 T , which developed cylindrical sporangia containing a longitudinal pair of motile spores forming singly or in bundles on short ramifications of the aerial mycelium, was isolated from soil collected from an evergreen forest in Thailand. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinones were MK-9(H 2 ). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannoside, diphosphatidylglycerol and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C 17 : 1 ,C 18 : 1 , saturated C 16 : 0 , and C 17 : 0 . The G+C content of the DNA was 70.8 mol%. Phenotypic and chemotaxonomic analyses showed that the isolate had the typical characteristics of members of the genus Planobispora. Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belonged to the genus Planobispora but as a putative novel species. DNA–DNA relatedness values that differentiate the isolate from previously described members of the genus Planobispora were significantly below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it is proposed that the isolate represents a novel species, Planobispora siamensis sp. nov.; the type strain is A-T 4600 T (5BCC 39469 T 5NBRC 107568 T ). The genus Planobispora was first proposed by Thiemann & Beretta (1968) and classified in the family Streptospo- rangiaceae by Goodfellow et al. (1990). It includes two recognized species: Planobispora longispora (Thiemann & Beretta, 1968) as the type species and Planobispora rosea (Thiemann, 1970). Cells are Gram-positive, non-acid-fast and aerobic. They develop cylindrical to clavate spore vesicles that contain a longitudinal pair of spores forming singly or in bundles on short ramifications of the aerial mycelium. Spores are motile by peritrichous flagella (Thiemann & Beretta, 1968). This genus has N-acetylated muramic acid and meso-diaminopimelic acid (A 2 pm) in the cell wall. It has madurose as whole-cell diagnostic sugar. Mycolic acids are not detected. The predominant menaqui- nones are MK-9(H 4 ), MK-9(H 2 ) and MK-9(H). The phospholipid pattern is type PIV. The DNA G+C content is 70–71 mol% (Ara & Kudo, 2007; Vobis, 1989). The genus Planobispora is closely related to the genus Planomonospora (Thiemann et al., 1967). Members of the two genera exhibit a high 16S rRNA gene sequence similarity, but can be distinguished from each other on the basis of morphological criteria, i.e. members of the genus Planomonospora develop single spores in cylindrical spore vesicles. During a study on the diversity of actinomycetes in Thailand, strain A-T 4600 T was isolated from soil in Kanchanaburi Province. The isolate was identified as a new member of the genus Planobispora based on morphological and chemotaxonomic studies, phylogenetic analysis and DNA–DNA relatedness studies. Here, we report the taxonomic characterization and classification of strain A- T 4600 T and propose a novel species as Planobispora siamensis sp. nov. to accommodate the strain. Abbreviation: A 2 pm, diaminopimelic acid. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Planobispora siamensis A-T 4600 T is HQ599197. Two supplementary figures and a supplementary table are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2013), 63, 2649–2654 DOI 10.1099/ijs.0.046946-0 046946 G 2013 IUMS Printed in Great Britain 2649