1 TITLE: Extracellular vesicle-mediated transfer of CLIC1 protein is a novel mechanism for the regulation of glioblastoma growth. AUTHORS: Setti M.* 1 , Osti D.* 1 , Richichi C. 1 , Ortensi B. 1 , Del Bene M. 2 , Fornasari L. 1 , Beznoussenko G. 3 , Mironov A. 3 , Rappa G. 4 , Cuomo A. 1 , Faretta M. 1 , Bonaldi T. 1 , Lorico A. 4 , Pelicci G. 1,5, AFFILIATIONS: 1. Department of Experimental Oncology, European Institute of Oncology, 20139 Milan, Italy; 2. Department of Neurosurgery, Fondazione IRCCS Istituto Neurologico C. Besta, via Celoria 11, 20133, Milan, Italy; 3. Institute of Molecular Oncology (IFOM) of the Italian Foundation for Cancer Research (FIRC), 20139 Milan, Italy; 4. Cancer Research Center, Roseman University of Health Sciences with Roseman University College of Medicine, Las Vegas, NV, USA; 5. Department of Translational Medicine, Università del Piemonte Orientale, Novara, Italy. * Equally contributors CORRESPONDING AUTHOR: Pelicci Giuliana, via Adamello 16, Department of Experimental Oncology, European Institute of Oncology 20139 Milan, Italy, giuliana.pelicci@ieo.eu KEYWORDS: Glioblastoma – Cancer stem cells – Extracellular vesicles – Cell proliferation – Tumor growth ABSTRACT Little progresses have been made in the treatment of glioblastoma (GBM), the most aggressive and lethal among brain tumors. Recently we have demonstrated that Chloride Intracellular Channel-1 (CLIC1) is overexpressed in GBM compared to