ƩƽƺǃƻƳ ǚ ƜǁǁǃƳ ǚ Ɲ ƧƷǁǁǃƳ ƦƱƷ ƘƼƵ ƜƦƦơ ƝƧƦƘ ƯƼ ƽƾƳƼ ƯƱƱƳǁǁ ƸƽǃǀƼƯƺ 5HVHDUFK $UWLFOH 2SHQ $FFHVV 7LVVXH 6FLHQFH (QJLQHHULQJ ƔƹƳǀưƳǀƵ Ƴǂ Ưƺ Ɲ ƧƷǁǁǃƳ ƦƱƷ ƘƼƵ ƶǂǂƾƲdžƲƽƷƽǀƵ Keywords: Abdominal adhesions; Prevention; Polypeptides; Adhesiolysis Introduction Abdominal adhesions constitute major health related problems, both for the individual patient, and globally due to large expenditures for the healthcare [1,2]. Most of the abdominal adhesions form due to previous peritoneal damage, mainly during abdominal surgery, and may end up causing intestinal obstructions, pain and female infertility, along with other complications [3,4]. e overall picture of how abdominal adhesions develop is quite clear, although some parts remain to be elucidated. Abdominal adhesions form as a result of peritoneal injury. Peritoneum is a serous delicate organ, lining the abdominal cavity, with both protective and restorative functions [5]. Peritoneum consists of a single layer of loosely attached mesothelial cells, resting on a basal lamina and a submesothelial area. e submesothelial area contains resident cells, capillaries and lymphatic vessels [6]. Mesothelial cell detachment is an immediate consequence of peritoneal damage of any kind, leading to serosanguinous leak in the area, forming a brin mesh between injured peritoneal sites. e brin is usually resolved due to local brinolysis, however during extensive trauma and local tissue hypoxia, the brin strands may persist, leading to stable adhesions through deposition of collagen and other extracellular matrix proteins [7-9]. One important factor initiating brinolysis via the serine protease plasmin in previous damaged peritoneum is the Tissue plasminogen activator (tPA), stored in great quantities in endothelial and mesothelial cells on the peritoneum [10]. Plasminogen activator inhibitor (PAI- 1), on the other hand, is an important factor impairing brinolysis through inhibition of tPA [11,12]. e production of collagen and other extracellular matrix proteins in damaged peritoneum is mostly governed by broblasts and active transforming growth factor beta 1(TGF-β1) [13]. Many attempts have been made during the past years to prevent postoperative adhesions, although none have been feasible in every aspect. e group has focused on combining positively charged polymers, Poly-L-lysine (PL) with negatively charged polymers, Poly- L-Glutamate (PG), forming a bioactive, nontoxic, degradable polymer that seals of the injured peritoneal site, prohibiting brin deposition and eventually, the development of postsurgical abdominal adhesions. Previous studies have focused on comparing the anti adhesion eects in the abdomen aer the primary surgery, when administering the PL/ PG complex [14-16]. However, a huge amount of patients undergoes repeated surgical procedures in the abdomen, causing extensive adhesions, which oen forces the surgeons to perform intra abdominal adhesiolysis, thereby increasing the risk of complications such as bleedings, stulas, abscesses and more [17-20]. In this study, the examination of the potential anti adhesion eect of PL/PG aer adhesiolysis was focused. 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