J. Nrurogmelics, IS: 75-95. 2001 Copyright '4," 2001 Taylor & Francis 0167-7063/01 $12.00 + .OO GENERATION OF A SEMI-DOMINANT MUTATION WITH TEMPERATURE SENSITIVE EFFECTS ON BOTH LOCOMOTION AND PHOTOTRANSDUCTION IN DROSOPHILA MELANOGASTER BRENDON 0. WATSON, ILYA VILINSKY and DAVID L. DEITCHER Department of Neurobiology and Behavior, Cornell University, Ithaca, N Y (Received 29 November 2000: uccepred 12 June 2001). A novel Drosophila mutant named Tripped-and-fell (Tad was isolated in a F1 screen for dominant temperature sensitive paralytics. Re- combination mapping using multiply marked chromosomes and P elements have pinpointed the locus of Taf to polytene band 93 on the right arm of the third chromosome (3R). When exposed to restrictive temperatures, both Taj' heterozygotes and homozygotes paralyzed; however, homozygotes paralyzed at lower temperatures and took longer to recover than heterozygotes. There are also positive correla- tions between recovery time from paralysis and both duration and tem- perature of exposure. Electroretinograms (ERGs) revealed that both homozygotes and heterozygotes have a grossly normal light response at 22"C, but at 37"C, the ERGs from both homozygotes and heterozygotes are unable to maintain a normal sustained depolarization and have a This research was funded by American Heart Association Development Grant (D.L.D.) and NIH Cell and Molecular Neurobiology Training Grant (5T32GM07469 to I.V.). The authors would like to thank Dr. Patricia Rivlin, Sujata Rao, Dr. Bryan Stewart, Dr. R. S. Stowers and Dr. Cole Gilbert for helpful discussions, and Jennifer Bestman for statistical advice. We would especially like to thank Dr. Ron Hoy and Dr. Elke Buschbeck for generously allowing us to use their electrophysiological recording and analysis equipment. Address correspondence to: David L. Deitcher, Department of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853. E-mail: dldl4@cornell.edu 75 J Neurogenet Downloaded from informahealthcare.com by Nyu Medical Center on 06/02/15 For personal use only.