Gene Section Review Atlas Genet Cytogenet Oncol Haematol. 2008;12(6) 421 Atlas of Genetics and Cytogenetics in Oncology and Haematology OPEN ACCESS JOURNAL AT INIST-CNRS CDC25A (cell division cycle 25A) Dipankar Ray, Hiroaki Kiyokawa Department of Mol. Pharmacol & Biol. Chem, Northwestern University, Chicago, IL 60611, USA (DR, HK); Robert H. Lurie Compre. Cancer Center, Northwestern University, Chicago, IL 60611, USA (HK) Published in Atlas Database: February 2008 Online updated version: http://AtlasGeneticsOncology.org/Genes/CDC25AID40004ch3p21.html DOI: 10.4267/2042/38593 This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. © 2008 Atlas of Genetics and Cytogenetics in Oncology and Haematology Identity Hugo: CDC25A Other names: CDC25A2 Location: 3p21 Local order: The gene is located telomeric to CAMP (cathelicidin antimicrobial peptide) and centromeric to LOC729349 (a pseudogene similar to 60S ribosomal protein L17 (L23)). The gene starts at 48,173,672 bp from pter and ends at 48,204,805 bp from pter with a total size of 31,133 bases. DNA/RNA Description CDC25A is about 31.13 kb located on the short (p) arm of chromosome 3, in the centromere-to-telomere orientation. The gene has 15 exons and the start codon is located at the end of exon 1 and stop codon in the beginning of exon 15. Transcription The CDC25A transcript is 3704 bp in length. So far there are two major transcript variant have been reported, CDC25A1 and A2. The transcript variant CDC25A2, has a deletion of 120 nucleotide (exon 6) resulted in a protein having truncation of 40 amino acid (between amino acid 143-182). However, both the N- terminal and C-terminal end of the protein is the same in both splice variant. Protein Description The full length CDC25A protein consists of 524 amino acids with an estimated molecular weight of 59 kDa. The other reported isoform CDC25A2, consists of 484 amino acids with a molecular weight of 54.4 kDa. Both the isoforms have the same N- and C-terminal end, thus expected to have similar catalytic activity. The N- terminal regulatory domain contains several phosphorylation sites and show low sequence homology between CDC25 family members, whereas C-terminal end has conserved Rhodanese homology domain containing the active site cysteine. The catalytic site contain the CX5R motif (C= cysteine; X= any amino acid; R= arginine) common to all protein tyrosine phosphatases. Genomic organization of human CDC25A gene on chromosome 3 p-ter.