_____________________________________________________________________________________________________ *Corresponding author: E-mail: profsakur@gmail.com; International Research Journal of Pure & Applied Chemistry 22(3): 34-42, 2021; Article no.IRJPAC.68162 ISSN: 2231-3443, NLM ID: 101647669 Novel Rapid Validated TLC- Densitometry for the Simultaneous Determination of Three Co-formulated Drugs used for Deep Tooth Inflammation Treatment Mohammad Marouf 1 , Ghoufran Kawas 1 and Amir Alhaj Sakur 2* 1 Department Chemistry, Faculty of Science, Tishreen University, Syria. 2 Department of Analytical and Food Chemistry, Faculty of Pharmacy, University of Aleppo, Syria. Authors’ contributions This work was carried out in collaboration among all authors. Author GK performed the experiments and wrote the first draft of the manuscript. Author AAS put the protocols for pharmaceutics and samples preparation, contributed to the interpretation of data and revised the manuscript. Author MM managed the project in all stages and revised the manuscript. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IRJPAC/2021/v22i330394 Editor(s): (1) Dr. Wolfgang Linert, Vienna University of Technology, Austria. Reviewers: (1) S. Parthasarathy, Mahatma Gandhi Medical College and Research Institute, India. (2) C. Kalaimagal, Bharathidasan University, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/68162 Received 01 March 2021 Accepted 05 May 2021 Published 13 May 2021 ABSTRACT A new use of a tertiary antibiotic combination of ciprofloxacin, clindamycin and metronidazole is currently being studied as an intracranial (intracranial) drug in an effort to disinfect the root canal system to revitalize the tooth with dead pulp. It is necessary to developing analytical method for the new drug combination. Herein we developed a rapid validated thin-layer chromatography (TLC)‒densitometric method has been developed for the simultaneous determination of 3 co-formulated drugs used for deep tooth inflammation Treatment. The studied drugs are Ciprofloxacin.HCL (CIP), Clindamycin phosphate (CLI) and Metronidazole(MET). The separation was achieved by using silica gel 60 F254 plates (20*20 cm) as stationary Phase and the developing system of Dichloromethane:n- Hexane:methanol:Ethylamine:Triethylamine (40:20:32:3:5)v/v. Densitometry scanning was performed at 220 nm. The R f values of CIP, CLI and MET were found Original Research Article