Open Peer Review Any reports and responses or comments on the article can be found at the end of the article. RESEARCH ARTICLE Renal corpuscle and tubule morphology in ephrin-A2 , ephrin-A5 and ephrin-A2A5 mice [version 1; peer review: 1 approved, 1 approved with reservations] Andrea E. Bertram , Robert J. Dugand , Clodagh Guildea , Samantha Lostrom , Gastor Lyakurwa , Alexandra Windsor , Marissa Penrose-Menz, Tom Stewart, James E. O’Shea, Jennifer Rodger School of Animal Biology, The University of Western Australia, Crawley, WA 6009, Australia Equal contributors Abstract The B family of Eph receptor tyrosine kinases and their ephrin ligands, best known for their role in the development of the nervous and vascular systems, have recently been implicated in mammalian kidney development and maintenance. However, the renal expression and function of the EphA and ephrin-A families have not been investigated. We performed immunohistochemistry for ephrin-A2 and ephrin-A5 in kidneys of normal adult wildtype (WT) mice and carried out quantitative morphological analysis of renal corpuscles and tubules in haematoxylin- and eosin-stained sections of WT, ephrin-A2 , ephrin-A5 and ephrin-A2A5 (knockout) mice. Ephrin-A2 and ephrin-A5 were strongly expressed in the tubules and glomeruli of the adult mouse kidney. Despite the significant overlap in expression between the two proteins, only the lack of ephrin-A5 had an effect on kidney morphology with glomerular size being mildly reduced in mice lacking the gene for ephrin-A5. However, the magnitude of this change was very small and could only be detected when animals were pooled across genotypes lacking ephrin-A5. The subtle phenotype, together with the relatively infrequent incidence of kidney failure in our breeding colony, suggest that ephrin-A2 and ephrin-A5 play only minor roles in kidney development and function. It is likely that other members of the ephrin-A family are expressed in the mouse kidney and redundancy within this large family of “promiscuous” signalling molecules may compensate for the loss of individual proteins in knockout mice. -/- -/- -/- * * * * * * * Reviewer Status Invited Reviewers version 1 published 11 Oct 2013 1 2 report report , Niigata University Graduate Hiroshi Kawachi School of Medical and Dental Sciences, Niigata, Japan 1 , Queensland Institute of Medical Andrew Boyd Research, Brisbane, Australia 2 11 Oct 2013, :212 ( First published: 2 ) https://doi.org/10.12688/f1000research.2-212.v1 11 Oct 2013, :212 ( Latest published: 2 ) https://doi.org/10.12688/f1000research.2-212.v1 v1 -/- -/- -/- Page 1 of 9 F1000Research 2013, 2:212 Last updated: 16 MAY 2019