94 Jurnal Sains Farmasi & Klinis p-ISSN: 2407-7062 | e-ISSN: 2442-5435 homepage: http://jsfk.ffarmasi.unand.ac.id Introduction The most effective antidiarrheal drugs are the opioids and derivatives of opioids that have been selected for maximal antidiarrheal and minimal central nervous system effect. Of the latter group, the most important are diphenoxylate and loperamide, meperidine analogs with weak analgesic effects. Diphenoxylate is formulated with antimuscarinic alkaloids (e.g., atropine) to reduce the likelihood of abuse; loperamide is formulated alone [1]. Loperamide hydrochloride has molecular formula C29H33ClN2O2.HCl and molecular weight 513,51 g/mol. Chemically, loperamide hydrochloride is 4-(p-Chlorophenyl)-4-hydroxy-N,N-dimethyl-α,α- diphenyl-1-piperidine butyl amide monohydrochloride [34552-83-5] as presented in Figure 1. The physicochemical properties for loperamide hydrochloride are powder; white to slightly yellow; melt at a temperature of approximately 225 oC with decomposition; readily soluble in methanol, in isopropyl alcohol and chloroform; challenging to dissolve in water and dilute acid [2]. Loperamide hydrochloride in the form of pharmaceutical raw materials can be determined by the method of water-free titration, whereas in the form of pharmaceutical preparations of tablets and capsules can be determined with high-performance liquid chromatography [2-4]. A quantitative method using silica gel high-performance thin layer chromatography plates with fuorescent indicator, automated sample application, and automated ultraviolet absorption densitometry of the fuorescence quenching zones was developed and validated for the determination of loperamide hydrochloride in anti-diarrheal medications [5]. Two new loperamide potentiometric electrodes were prepared and used for pharmaceutical analysis [6]. Two simple, economical, precise and reproducible visible spectrophotometric methods have been developed for the estimation of loperamide hydrochloride in tablet formulation [7, 8]. Among the various methods used in the determination of drug levels, UV-Vis spectrophotometry is still very popular. In our previous research, we have developed several analytical methods using the absorption method and the area measurement method under the curve with ultraviolet-visible spectrophotometry [9 - 16]. In Access this artcle ORIGINAL ARTICLE Vol. 5 No. 2 (Agustus 2018) | pp. 94–101 | Development and Validation of Loperamide Hydrochloride Tablet Analysis Method with Absorbance and Area under Curve Methods Spectrophotometrically Harrizul Rivai 1* , Renny Puspita 2 , & Sesry Misfadhila 2 1 Faculty of Pharmacy, Andalas University 2 School of Pharmaceutcal Science Kurao ABSTRACT: Development and validaton of loperamide hydrochloride tablet method of analysis have been done by using the absorbance method and area under the curve method. This study used methanol and hydrochloric acid 0.1 N (9: 1) as the best solvent with maximum absorpton at wavelength 259.00 nm. The linearity of loperamide hydrochloride was obtained in the concentraton range 200-600 ppm with a correlaton coefcient value with the absorbance method and the areas under the curve method were 0.9998 and 0.9865, respectvely. The results showed that the levels of loperamide hydrochloride in generic tablets obtained by absorbance method and the area under the curve method were 105.71% and 96.20%, respectvely. The results of determinaton of loperamide levels in tablets of trademark obtained by absorbance method and the area under the curve method were 102.85% and 98.57%, respectvely. The level of both samples met the requirements of Pharmacopoeia Indonesia editon V that is 90% -110%. Keywords: Loperamide hydrochloride; absorbance method; area under the curve method; spectrophotometry; development; validaton; analysis. *Corresponding Author: Harizul Rivai Fakultas Farmasi, Universitas Andalas, Jalan Universitas Andalas, Limau Manis, Pauh, Kota Padang, Sumatera Barat 25163| Email: harrizul@yahoo.co.id