S1 Supporting Information Water soluble AIE-gen for organic-solvent-free detection and wash-free imaging of Al 3+ ions and subsequent sensing of F - ions and DNA tracking Sharanabasava D. Hiremath, a Ram U. Gawas, a Starlaine C. Mascarenhas, b Anasuya Ganguly, b Mainak Banerjee, *,a Amrita Chatterjee *,a a Department of Chemistry, BITS, Pilani- K. K. Birla Goa Campus, NH 17B Bypass Road, Zuarinagar, Goa 403726, INDIA. b Department of Biological Sciences, BITS, Pilani- K. K. Birla Goa Campus, NH 17B Bypass Road, Zuarinagar, Goa 403726, INDIA. Table of Contents 1. Materials and methods...…………………………………………………..................S2-S3 2. Synthesis of sodium 4-(4-(1,2,2-triphenylvinyl)-phenoxy)butane-2-sulfonate (TPE- diBuS)………...………………………………………………………………………....S4 3. Aqueous solubility study of TPE-diBuS………………………………………………..S5 4. pH study …………………………………………………………………………...….....S5 5. Association constant calculation…………………………………………………….......S6 6. DLS Study ………………………………………………………………………………S6 7. EDX chart …………………………………………………………………...…...……...S7 8. EDTA response towards TPE-diBuS-Al ensemble….…………………………….........S7 9. DFT optimized structures of metal-TPE-BuS complexes ……………………..……S8-S9 10. Comparison table of Al-probes with TPE-diBuS..………………...……………............S9 11. Limit of quantitation (LOQ) of Al 3+ ions …………………………………..……..…...S10 12. Real sample analysis for Al 3+ ions ………………………………….....……………….S10 13. Limit of detection (LOD) of F - ions……………………………………………………S11 14. Comparison table of other metal-ensemble based F - sensors with Al-TPE-diBuS…....S11 15. Selectivity of anions by TPE-diBuS-Al ensemble ………………………...…………S12 16. Cytotoxicity data ………………………………………………………………..……..S12 17. Wash free imaging of Al 3+ ions…….………………………………………………..…S13 18. References…………………………………………………………………………..…..S13 19. NMR and HRMS spectra TPE-diBuS………………………….…………………S14-S15 Electronic Supplementary Material (ESI) for New Journal of Chemistry. This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2019