2 A novel bio-electronic tongue using different cellobiose dehydrogenases to resolve mixtures of various sugars and interfering analytes Andrea Cipri* 1 ; Christopher Schulz* 2 ; Roland Ludwig 3 ; Lo Gorton 2 ; Manel del Valle 1 ; 1 Sensors & Biosensors Group, Chemistry Department, Universitat Autònoma de Barcelona, Barcelona, Spain; 2 Department of AnalyticalChemistry/Biochemistry and Structural Biology, Lund University, Lund, Sweden; 3 Department of Food Science and Technology BOKU – University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria *Both authors contributed equally to this work Abstract A novel application of cellobiose dehydrogenase enzyme (CDH) as sensing element for an Electronic Tongue (ET) system has been tested. In this work CDH from various fungi, which exhibit different substrate specificities were used to discriminate between lactose and glucose in presence of the interfering matrix compound Ca 2+ in various mixtures. This work exploits the advantage of an ET system with practically zero pre-treatment of samples and operation at low voltages in a direct electron transfer mode. The Artificial Neural Network (ANN) used in the ET system to interpret the voltammetric data was able to give a good prediction of the concentrations of the analytes considered. The correlation coefficients were high, especially for lactose (R 2 = 0.975) and Ca 2+ (R 2 = 0.945). This ET application has a high potential especially for the food and dairy industry and also, in a future miniaturized system, for in situ food analysis. Keywords : bioelectronic tongue; voltammetry; artificial neural networks; cellobiose dehydrogenase; glucose; lactose; calcium