complementary treatment, with low calorie diet on weight loss and Leptin hormone level, in Iranian overweight and obese subjects. Materials and methods: In the study subjects were approached on the overweight and obese persons, aged between 18 and 55 years with body mass index (BMI) between 26 and 45 kg/m 2 and who had medical history without drug history within the last 3 months. The subjects were randomly (by case to case option) divided to two groups. The case group (n = 43, female = 33, male = 10) received Electro Acupuncture with low-calorie diet, and the control group (n = 43, female = 33, male = 10) received sham Electro Acupuncture with low-calorie diet. All subjects were asked to receive twice treatments each week for 6 weeks and needles were kept on the body for 20 min in each time. Body weight, BMI, body fat mass and leptin hormone were measured two times in all subjects (first; at the beginning of study, second; 1 day after treatment). Results: Body weight and BMI, decreased significantly (p <0.001) in both the case and control groups but the difference of these factors between two group was not statistically significant (p > 0.1). For body fat mass and leptin hormone, in the case group it was observed that there was a significant reduction in the two variables (p<0.001, p<0.001), respectively and in the control group significant reduction in body fat mass (p <0.005) and leptin hormone (p < 0.01) was observed. The difference of these factors between the two groups was not statistically significant in body fat mass (p > 0.1) and was significant in leptin hormone (p < 0.03). Conclusion: This study found no statistical difference in body weight and BMI (mean± S.D), between the two groups. Electro acupuncture may have potential benefit on body fat mass and leptin hormone. Keywords: Electro acupuncture, Obesity, Overweight, Body weight, BMI, Body fat mass, Leptin hormone doi:10.1016/j.clinbiochem.2011.08.301 Poster — [A-10-94-1] The effects of auricular acupuncture on leptin hormone in Iranian obese and overweight subjects Sara Darbandi a , Mahsa Darbandi a , Majid Ghayour Mobarhan b , Pooneh Mokaram a , Ali Akbar Owji a , Mehdi Saberfiroozi b , Hamid Abdi b , Mohsen Nematy b , Mohamad Safarian b , Mohamad Reza Parizadeh b , Parisa Abbasi b , Baxiao Zhao b , Gordon Ferns c a Shiraz, Iran b Mashhad, Iran c Surrey, UK E-mail addresses: mahsasaraa@yahoo.com (S. Darbandi), ghayourM@mums.ac.ir (M. Ghayour Mobarhan), mokaram2@gmail.com (P. Mokaram), aaowji@yahoo.com (A.A. Owji), abdi13462000@yahoo.com (H. Abdi), nematyM@mums.ac.ir (M. Nematy), safarianM@mums.ac.ir (M. Safarian), ParizadehMR@mums.ac.ir (M.R. Parizadeh), mr_d@yahoo.com (P. Abbasi), g.a.a.ferns@istm.keele.ac.uk (G. Ferns) Introduction: In the current study we have evaluated the effects of auricular acupuncture, as a kind of complementary treatment, with low calorie diet on weight loss and leptin hormone level. Materials and methods: In this study subjects were approached on the overweight and obese persons, aged between 18 and 55 years with body mass index (BMI) between 26 and 45 kg/m 2 and who had medical history without drug history within the last 3 months. The subjects were blinded and randomly (by case to case option) divided to two groups. The case group (n = 43, female = 38, male = 5) received auricular acupuncture with low-calorie diet, and the control group (n = 43, female = 38, male = 5) received sham auricular acupuncture with low-calorie diet. In the two groups, needles were replaced twice each week for 6 weeks. Body weight, BMI, body fat mass and leptin hormone were measured two times in all subjects (first; at the beginning of study, second; 1 day after treatment). Results: Body weight and BMI, decreased significantly (p< 0.001) in both case and control groups but the difference of these factors between the two groups was not statistically significant (p>0.1). However, it was observed that there was a significant reduction in body fat mass (p < 0.05) and plasma leptin hormone (p < 0.01) in the case group with no significant changes (p >0.1) in control group. Conclusion: This study found no statistical difference in body weight and BMI (mean± S.D), between the two groups. Auricular acupuncture may have potential benefit on body fat mass and leptin hormone. Keywords: Auricular acupuncture, Obesity, Overweight, Body weight, BMI, Body fat mass, Leptin hormone doi:10.1016/j.clinbiochem.2011.08.302 Poster – [A-10-95-1] Evaluation of Melatonin effects on some biochemical parameters In rainbow trout (oncorhynchus mykiss) Hamideh Ghodrati Azadi a , Hasan Baghishani b , Davar Shahsavani b a Mashhad University of Medical sciences, Mashhad, Iran b Department of Faculty of Veterinary medicine E-mail addresses: ghodrati@mum.ac.ir (H. Ghodrati Azadi), baghishani@ um.ac.ir (H. Baghishani), shahsavani@ um.ac.ir (D. Shahsavani) Introduction: Much of melatonin in special nerve cells (pineal) to a small gland in the center of the brain is made. Dark strengthen and intensify construction of melatonin and light, it will prevent the discharge. The purpose of this experiment was to determine the effect of long–term low–dose administration of Melatonin (MLT) as an antioxidant in fish. Materials and Methods: In this study, 90 carp weighted average number of 60 to 70 grams, were randomly allocated into the control (n=45) and Melatonin (MLT) - treated (n=45) groups. They were distributed and spent a week to become compatible with the environment. To ensure the health of fish parasites and microbial assay in a number of them were done. To achieve the desired goals during the testing of a particular type of diet for each of the two groups were used. The first groups 10 mg/kg MLT to their dry food (3% body weight) added (4 weeks) and for the control diet is considered normal. Both groups were kept under identical conditions in terms of food and environment. Blood samples were drawn between the hours of 08.00 and 09.00 from the both groups on days 0, 7, 14, 21 and 28. Anti oxidant effect was evaluated by measuring the levels of the serum glutathione peroxidase, (GPX) Superoxide dismutase (SOD), Malondialdehyde (MDA) and catalase. Hepatic function was evaluated in both groups by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST). Results: MLT increased activity of antioxidative enzymes and decreased peroxidation of lipids, when compared to the control group (p < 0.05). Conclusion: It was concluded that MLT administration have antioxidative effects. Keywords: Melatonin, Biochemical parameters, Rainbow trout doi:10.1016/j.clinbiochem.2011.08.303 Abstracts S128