Research Article AntioxidantandAnti-InflammatoryActivitiesof Kigelia africana (Lam.) Benth. Alice Nabatanzi , 1,2,3,4,5 Sanah M. Nkadimeng, 2 Namrita Lall, 3,6,7 John D. Kabasa, 4 and Lyndy J. McGaw 2 1 Department of Plant Sciences, Microbiology and Biotechnology, College of Natural Sciences, Makerere University, Kampala 00256, Uganda 2 Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa 3 Department of Plant and Soil Sciences, University of Pretoria, Hatfield 0028, South Africa 4 College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala 00256, Uganda 5 Future Africa, University of Pretoria, Hatfield 0028, South Africa 6 School of Natural Resources, University of Missouri, Columbia 65211, USA 7 College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570015, India Correspondence should be addressed to Alice Nabatanzi; alice2nabatanzi@gmail.com Received 23 March 2020; Accepted 5 May 2020; Published 17 June 2020 Academic Editor: Armando Zarrelli Copyright © 2020 Alice Nabatanzi et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Kigelia africana is used to manage inflammation among indigenous communities. We hypothesized that K. africana extracts contain phytoconstituents with good antioxidant and anti-inflammatory activities. e methanolic extract of K.africana fruits and Spathodeacampanulata leaves (SPK04), K.africana aqueous fruit extract (KFM02), and K.africana acetone fruit extract (KFM05) were subjected to antioxidant and anti-inflammatory assays. Antioxidant activity was evaluated using the ABTS radical scavenging assay, and the MTTcell viability assay was used for cytotoxicity. e extracts were preincubated with enzymes and assayed for 15- LOX and COX-2 enzyme activity using an ELISA method. Nitric oxide (NO) inhibitory effect of the extracts was evaluated and measurement of proinflammatory cytokines (IL-1β, TNF-α, and IL-6) and the anti-inflammatory cytokine (IL-10) was done using ELISA kits. SPK04 had the highest antioxidant activity with a mean inhibition of 99.37 ± 0.56% and an IC 50 of 4.28 µg/mL. SPK04 and KFM05 did not inhibit 15-LOX as their IC 50 values were >1000 μg/mL. All extracts were safe on Vero cells at the highest concentration (200 µg/mL) tested. KFM02 was the best inhibitor of NO production and had the highest cell viability at both the lowest (50 µg/mL) and highest concentrations (200 µg/mL). SPK04 was the best COX-2 inhibitor while KFM05 expressed the strongest suppression effect for IL-β and IL-6. KFM02 did not inhibit IL-6 at the highest concentration (200 µg/mL). e order of suppression of TNF-α by the extracts differed across concentrations, KFM05 > SPK04 > KFM02 at 200 µg/mL, KFM02 > SPK04 > KFM05 at 100 µg/mL, and SPK04 > KFM02 > KFM05 at 50 µg/mL. All the tested extracts had no inhibitory effect against IL-10. SPK04, KFM05, and KFM02 had good antioxidant and anti-inflammatory activity and this supports their use as potential anti-inflammatory therapies. is study presents for the first time the antioxidant and anti-inflammatory activity of K. africana and S. campanulata polyherbal extract. It is also among the very few studies that have reported the inhibitory effect of cytokines IL-1β, TNF-α, IL-6, and IL-10 by K. africana. 1. Introduction Inflammation is the body’s first protective mechanism to enable tissue healing when faced with injurious stimuli [1]. e body responds to inflammation by releasing proin- flammatory cytokines such as interleukins (IL-1β and IL-6), interferons (IFN), and tumor necrosis factor-alpha (TNF-α) among others [2]. is is followed by induction of Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 4352084, 11 pages https://doi.org/10.1155/2020/4352084