Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Fri, 14 Jun 2019 12:12:18 Nitrincola alkalilacustris sp. nov. and Nitrincola schmidtii sp. nov., alkaliphilic bacteria isolated from soda pans, and emended description of the genus Nitrincola Andrea K. Borsodi, 1, * Kristóf Korponai, 1 Peter Schumann, 2 Cathrin Spröer, 2 Tam as Felföldi, 1 K aroly M arialigeti, 1 Tibor Szili-Kov acs 3 and Erika Tóth 1 Abstract Three alkaliphilic and halotolerant bacterial strains, designated ZV-19 T , R4-8 T and S4-12, were isolated from the water of soda pans located in the Kiskuns ag National Park, Hungary. Cells of all three strains were Gram-staining-negative, rod- shaped, motile and non-endospore-forming. They were facultatively anaerobic, and oxidase- and catalase-positive. Their major isoprenoid quinone was Q-8, and their predominant fatty acids were C 18 : 1 !7c,C 16 : 1 !7c and C 16 : 0 . The DNA G+C content was 54.5 mol% in strain ZV-19 T and 45.8 mol% in strain R4-8 T . The 16S rRNA gene based phylogenetic analysis showed that all three strains were members of the genus Nitrincola (family Oceanospirillaceae, class Gammaproteobacteria). Strain ZV-19 T showed 96.6 and 95.5 % sequence similarities and 19±3 and 18±3 % DNA–DNA relatedness to Nitrincola lacisaponensis DSM 16316 T and Nitrincola alkalisediminis JCM 19317 T , respectively. Strains R4-8 T and S4-12 exhibited 97.9 and 98.6 % sequence matches and 34±4 and 13±8 % DNA–DNA hybridization values with N. lacisaponensis DSM 16316 T and N. alkalisediminis JCM 19317 T , respectively. According to the phenotypic, chemotaxonomic and phylogenetic data, the strains studied represent two novel species, Nitrincola alkalilacustris sp. nov. with the type strain ZV-19 T (=DSM 29817 T =NCAIM B 02612 T ) and Nitrincola schmidtii sp. nov. with the type strain R4-8 T (=DSM 100788 T =NCAIM B.02626 T ). An emended description of the genus Nitrincola is also presented. The genus Nitrincola (family Oceanospirillaceae, class Gammaproteobacteria) was described by Dimitru et al. [1] to include non-pigmented, asporogenous, motile, Gram- staining-negative, rod-shaped cells of oxidase- and catalase- positive, non-fermenting, facultatively anaerobic, chemo- organotrophic, alkaliphilic bacteria. The type strain of the species Nitrincola lacisaponensis was isolated from Soap Lake, a meromictic soda lake in the Rocky Mountains (North America) [1]. Recently, two other species, Nitrincola alkalisediminis [2] and ‘Nitrincola nitratireducens’ [3] were described from Lonar Lake, an alkaline crater lake in the Deccan Plateau (India). At present, draft genome sequences are available for Nitrincola strain A-D6, an arsenic-resistant bacterium isolated from a Chilean salt flat [4], and the strains DSM 16316 T of N. lacisaponensis [1] and AK23 of ‘N. nitratireducens’ [3], an effectively but not validly pub- lished species name. In addition, members of the genus Nitrincola have been isolated frequently from haloalkaline soda lakes located worldwide in the warm arid and semi- arid areas [5–9]. The present study describes the polyphasic taxonomic char- acterization of strains ZV-19 T , R4-8 T and S4-12 cultivated from planktonic bacterial communities of Hungarian soda pans. Strain ZV-19 T was isolated from the water of Zab-sz ek (46  50.190¢ N 19  10.283¢ E) while strains R4-8 T and S4-12 were isolated from a thin layer of a purple bacterial bloom occurring in a soda pan near Soltszentimre (46  45.818¢ N 19  10.828¢ E). Both sampling sites can be found in the Kiskuns ag National Park (Hungary), approximately 8 km from each other. Morphometric, physical and chemical characteristics of these water bodies were described by Boros et al. [10]. Author affiliations: 1 Department of Microbiology, ELTE Eötvös Lor and University, P azm any P eter s et any 1/C, H-1117 Budapest, Hungary; 2 Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7 B, 38124 Braunschweig, Germany; 3 Institute for Soil Sciences and Agricultural Chemistry, Agricultural Research Center, Hungarian Academy of Sciences, Herman Ottó út 15, H-1022 Budapest, Hungary. *Correspondence: Andrea K. Borsodi, borsodi.andrea@ttk.elte.hu Keywords: Nitrincola; alkaliphilic bacterium; soda lake. Abbreviations: MALDI-TOF MS, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry; ML, maximum-likelihood; NJ, neigh- bour-joining; RAPD, random amplification of polymorphic DNA. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strains ZV-19 T , R4-8 T and S4-12 are LN650603, KR233209 and KR233223, respectively. One supplementary table and five supplementary figures are available with the online version of this article. TAXONOMIC DESCRIPTION Borsodi et al., Int J Syst Evol Microbiol 2017;67:5159–5164 DOI 10.1099/ijsem.0.002437 002437 ã 2017 IUMS 5159