Indian Phytopath. 57 (4) : 497-498 (2004) Biochemical changes in host by carbendazim resistant Gloeosporium ampelophagum causing anthracnose of grape V.C. KHILARE 1 , A.B. ADE, A.S. DEOKATE 2 and L.V. GANGAWANE Soil Microbiology and Pesticides Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431 004 1 Botany Research Centre, Department of Botany, Vasantrao Naik Mahavidyalya, CIDCO, Aurangabad 431 003. 2 Chirayu Biotech, Champagne Vineyards Ltd., A/P. Narayangaon, Pune 415 504 Key words: Carbendazim, Gloeosporium ampelophagum, grape Anthracnose of grape (Vitis vinifera L.) caused by Gloeosporium ampelophagum (Pass.) Sacc. is an important disease observed in Maharashtra. The pathogen infects the foliage, petiole, twigs and berries. The fungicide carbendazim is recommended in the management of this disease (11). However, carbendazim resistance in Gloeosporium ampelophagum has been observed (2,4). Very few reports are available on the biochemical analysis of host due to infection by fungicide resistant and sensitive pathogen (6, 9). In this investigation an attempt has been made to find out the biochemical changes in different parts of grape infected by carbendazim resistant and sensitive isolates of G. ampelophagum. Altogether 210 isolates of G. ampelophagum were isolated from grape growing pockets of Maharashtra and maintained on Czapek Dox agar medium. Among them 87.61 %( resistance factor 1.07 to 2.55) isolates were sensitive and 12.38 % (resistance factor 4.20 to 4.93) were in resistant response category. Variety Thompson Seedless grape was used in the study. Carbendazim resistant (GA-35) and sensitive (GA-1) isolates were selected from earlier studies (4). These isolates were grown on Czapek-Dox liquid medium for seven days. Different plant parts like leaves, petioles, twigs and berries were inoculated with spore suspension by prick-point method. They were then incubated at laboratory temperature (26±3°C). After two weeks of incubation period infested parts were used for extraction. The extracts were used for biochemical analysis. Altogether nine parameters were considered for analysis viz, total chlorophyll (7), total sugars (5), total amino acids (3), total proteins (8), Ortho dihydric phenols (5), total phenols (1,5), DNA and RNA (10). Variations were observed in all the biochemicals studied in infected parts like leaves, petioles, twigs and berries with carbendazim resistant and sensitive G.ampelophagum as shown in Table 1. Total chlorophyll including chlorophyll - a and b was reduced drastically in resistant isolate. Total sugars, total amino acids, total proteins, DNA and RNA decreased in their quantity due to infection by both the isolates. This decrease was found to be more due to resistant isolate when compared with healthy tissue. Ortho dihydric phenols and total phenols increased due to resistant isolate. Increased production of amino acids in Hg and Captan resistant isolates of Macrophomina phaseolina has been noted by Rana and Sengupta (9) supporting this work. ACKNOWLEDGEMENT One of the authors (VCK) is thankful to The Principal, Vasantrao Naik Mahavidyalya, Aurangabad for his kind cooperation.