RESOURCE The Arabidopsis TAC Position Viewer: a high-resolution map of transformation-competent artificial chromosome (TAC) clones aligned with the Arabidopsis thaliana Columbia-0 genome Yoshitsugu Hirose 1 , Kunihiro Suda 2 , Yao-Guang Liu 3,4 , Shusei Sato 2,5 , Yukino Nakamura 6 , Koji Yokoyama 6 , Naoki Yamamoto 2,6,† , Shigeru Hanano 2, *, Eiji Takita 2,‡ , Nozomu Sakurai 2 , Hideyuki Suzuki 2 , Yasukazu Nakamura 2,§ , Takakazu Kaneko 2,¶ , Kentaro Yano 6 , Satoshi Tabata 2 and Daisuke Shibata 2, * 1 Honda Research Institute Japan Co., Ltd., 8-1 Honcho, Wako-shi, Saitama 351-0188, Japan, 2 Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan, 3 Laboratory of Genetic Engineering, South China Agricultural University, Gungzhou 510642, China, 4 State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural Univer- sity, Gungzhou 510642, China, 5 Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai 980-8577, Japan, and 6 Bioinformatics Laboratory, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kanagawa, Kawasaki 214-8571, Japan Received 7 April 2015; revised 13 July 2015; accepted 17 July 2015; published online 30 July 2015. *For correspondence (e-mails hanano@kazusa.or.jp; shibata@kazusa.or.jp) Accession numbers: GA919428 to GA933004. † Present address: Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO 7777, Metro Manila 1301, Philippines. ‡ Present address: Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd, 1280 Kami-izumi, Sodegaura, Chibta 299-0293, Japan. § Present address: Genome Informatics Laboratory, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan. ¶ Present address: Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigano, Kita-ku 603-8555, Japan. SUMMARY We present a high-resolution map of genomic transformation-competent artificial chromosome (TAC) clones extending over all Arabidopsis thaliana (Arabidopsis) chromosomes. The Arabidopsis genomic TAC clones have been valuable genetic tools. Previously, we constructed an Arabidopsis genomic TAC library consisting of more than 10 000 TAC clones harboring large genomic DNA fragments extending over the whole Ara- bidopsis genome. Here, we determined 13 577 end sequences from 6987 Arabidopsis TAC clones and mapped 5937 TAC clones to precise locations, covering approximately 90% of the Arabidopsis chromo- somes. We present the large-scale data set of TAC clones with high-resolution mapping information as a Java application tool, the Arabidopsis TAC Position Viewer, which provides ready-to-go transformable genomic DNA clones corresponding to certain loci on Arabidopsis chromosomes. The TAC clone resources will accelerate genomic DNA cloning, positional walking, complementation of mutants and DNA transfor- mation for heterologous gene expression. Keywords: Arabidopsis thaliana, transformation-competent artificial chromosome, Arabidopsis TAC clones, Arabidopsis TAC library, Arabidopsis genome, genomics, bioinformatics, GBrowse. INTRODUCTION The complete genomic sequence of Arabidopsis thaliana (Arabidopsis) was published in 2000, and was the first to be determined in the plant kingdom (Arabidopsis Genome Initiative, 2000). Recent rapid progress in DNA sequencing technology has also revealed genome sequences from many other plant species (Hamilton and Buell, 2012; © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd 1114 The Plant Journal (2015) 83, 1114–1122 doi: 10.1111/tpj.12949