Ravisankar Periyasamy, Ramesh Kumar, Ravindran Rajan, Omprakash Kasaragod. Neurochemical Observations after the Transplantation of Human Amniotic Epithelial Cells in 2, 4, 5-trihy- droxyphenylethylamine (6-OHDA) induced Parkinsonism disease model in Wistar albino rats. Int J Anat Appl Physiol. 2021;07(01):164-173. 164 OPEN ACCESS https://scidoc.org/IJAAP.php Neurochemical Observations after the Transplantation of Human Amniotic Epithelial Cells in 2, 4, 5-trihydroxyphenylethylamine (6-OHDA) induced Parkinsonism Disease Model in Wistar albino rats Research Article Ravisankar Periyasamy 1* , Ramesh Kumar 2 , Ravindran Rajan 3 , Omprakash Kasaragod 4 1 Department of Anatomy, SRM.Dental College & Hospital, SRM Institute of Science and Technology, Ramapuram Campus, Bharathi Salai, Chennai. 2 Department of Anatomy, Dr. A. L. Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Taramani, Chennai. 3 Department of Physiology, Dr. A. L. Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Tarama- ni, Chennai. 4 Department of Anatomy, Hassan Institute of Medical Sciences, Hassan—573201, Karnataka, India. International Journal of Anatomy and Applied Physiology (IJAAP) ISSN: 2572-7451 Introduction Parkinson′s disease (PD) is one of the most common neurode- generative movement disorders. The most marked pathological change in this disease is selective degeneration of the nigrostri- atal dopaminergic neurons in the central nervous system [1]. The anatomical and functional changes in PD may be classifed into a three phase model explained by Peran et al., (2010) [2]. There are at least two possible explanations for the elevated DA turnover in residual neurons after partial lesions of dopaminergic projection. First one is the increase of DA which is mediated by a change in the fring of the remaining dopaminergic neurons. Indeed chang- *Corresponding Author: Ravisankar Periyasamy, Department of Anatomy, S.R.M. Dental College & Hospital, Bharathi Salai, Ramapuram Campus, Chennai -600089 Tel: +91 44 22490526 / +919444014570 E-mail: ravi_anat@yahoo.com Received: November 16, 2020 Accepted: Februay 06, 2021 Published: February 20, 2021 Citation: Ravisankar Periyasamy, Ramesh Kumar, Ravindran Rajan, Omprakash Kasaragod. Neurochemical Observations after the Transplantation of Human Amniotic Epithelial Cells in 2, 4, 5-trihydroxyphenylethylamine (6-OHDA) induced Parkinsonism disease model in Wistar albino rats. Int J Anat Appl Physiol. 2021;07(01):164-173. doi: http://dx.doi.org/10.19070/2572-7451-2100031 Copyright: Ravisankar Periyasamy © 2021. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. Abstract Background: In the present work the dopamine (DA) denervated rats exhibited vigorous contralateral rotational behaviour following apomorphine hydrochloride (APO Hcl) treatment. The 2, 4, 5-trihydroxyphenylethylamine (6-OHDA) lesioned animals showed signifcantly increased contralateral rotation. From the frst week onwards the researcher observed changes in the lesion-induced rotational behaviour with the dopaminergic agnostic drug APO Hcl for ffty-sixty minutes from the time of administration. The lesioned animals showed more than 25.40 folds of rotations were taken for the Human Amniotic Epithelial (HAE) cells transplantation. Objective: To study the role of HAE cells in rotational behaviour and the level of dopamine and its metabolites indiscrete regions of the brain in 6-OHDA lesionedwistar albino rats. Material and Methods: Total of 48 Adult male Wistar albino rats weighing 180-220 gms were used for this study and were divided into four groups. They were maintained in a room at constant temperature and humidity (21ºC to 26ºC) and 12h light and dark cycle during the behavioural test and surgery. The DA and its metabolites were studied usinghigh-performance liquid chromatography with electrochemical detection (HPLC- ECD) method. Results: The HAE cells transplanted animals showed decreased contralateral rotation for frst week 96%, second week 97%, third week 98% and fourth week 97% over the 6-OHDA lesioned animals. The level of DA and its metabolites in these ani- mals were signifcantly increased in transplanted side than the contralateral side of 6-OHDA lesioned animals. The maximum signifcant increase was observed in striatum (DA 44.5 fold, 3,4-Dihydroxyphenylacetic acid (DOPAC) 44.9 fold and homo- vanillic acid (HVA) 1.9 folds) and minimum increase was in cerebral cortex (DA 30 %, DOPAC 2 fold and HVA 2 fold) among the brain regions studied after the HAE cells graft from the 6-OHDA lesion. Conclusion: Increase of DA and its metabolites in brain region after the HAE cells graft may therefore probably be depend- ent upon the diffusion of certain neurotrophic factors in the region of graft in the striatum and also due to intimacy of contact between the graft and the host striatum. Keywords: 6-OHDA Lesion; Apomorphine Induced Rotations; Human Amniotic Epithelial (HAE) Cells; Dopamine (DA); 3,4-dihydroxyphenylacetic Acid ( DOPAC); Homovanillic Acid (HVA).