Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Thu, 13 Dec 2018 05:28:21 Pseudomonas salegens sp. nov., a halophilic member of the genus Pseudomonas isolated from a wetland Mohammad Ali Amoozegar, 1 Azadeh Shahinpei, 2 Abbas Akhavan Sepahy, 2,3 Ali Makhdoumi-Kakhki, 4 Shima Sadat Seyedmahdi, 2 Peter Schumann 5 and Antonio Ventosa 6 Correspondence Mohammad Ali Amoozegar amoozegar@ut.ac.ir or amoozegar@ibrc.ir 1 Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran 2 Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran 3 Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran 4 Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran 5 Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 6 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain A novel Gram-stain-negative, aerobic, non-endospore-forming, non-pigmented, rod-shaped, slightly halophilic bacterium, designated GBPy5 T , was isolated from aquatic plants of the Gomishan wetland, Iran. Cells of strain GBPy5 T were motile. Growth occurred with between 1 and 10 % (w/v) NaCl and the isolate grew optimally with 3 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 8.0 and 30 6C, respectively, while it was able to grow over a pH range of 6.5–9.0 and a temperature range of 4–35 6C. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain GBPy5 T is a member of the genus Pseudomonas forming a monophyletic branch. The novel strain exhibited 16S rRNA gene sequence similarity of 95.4 % with type strains of Pseudomonas guariconensis PCAVU11 T and Pseudomonas sabulinigri J64 T , respectively. The major cellular fatty acids of the isolate were C 18 : 1 v7c (37.8 %), C 16 : 0 (14.9 %), C 16 : 1 v7c (12.9 %), C 12 : 0 3-OH (7.1 %) and C 12 : 0 (7.0 %). The polar lipid pattern of strain GBPy5 T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The G+C content of the genomic DNA of strain GBPy5 T was 59.2 mol%. On the basis of the phenotypic and phylogenetic data, strain GBPY5 T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salegens sp. nov. is proposed. The type strain is GBPy5 T (5IBRC-M 10762 T 5CECT 8338 T ). The genus Pseudomonas, which was first described by Migula (1894), accommodates a group of Gram-stain- negative bacteria that are non-endospore-forming, aerobic, motile and rod-shaped (Palleroni, 2005). Pseudomonads are well-known micro-organisms with versatile metabolic activity and have been isolated from various environments like soil, plants, freshwater, clinical specimens and marine habitats (Palleroni, 1992; Romanenko et al., 2005). The taxonomic position of members of the genus Pseudomonas has changed several times (Stanier et al., 1966; Palleroni & Doudoroff, 1972; De Ley, 1992; Anzai et al., 2000). At the time of writing (October 2013) the genus comprises 207 species with validly published names (www.bacterio.net/m/ marinobacter.html; Euze ´by, 1997). Several species of the genus Pseudomonas can tolerate NaCl concentrations of up to 8 % (w/v) and are considered to be halotolerant. For a few species, which were first described as members of the The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain GBPy5 T is KF830707. Three supplementary figures are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2014), 64, 3565–3570 DOI 10.1099/ijs.0.062935-0 062935 G 2014 IUMS Printed in Great Britain 3565