Olivia A Hanafah et al 296 wjd Quantity Differences of Secondary Metabolites (Saponins, Tannins, and Flavonoids) from Binahong Plant Extract (Anredera cordifolia (Ten.) Steenis) treated and untreated with Colchicines that play a Role in Wound Healing 1 Olivia A Hanafah, 2 Diana S Hanafah, 3 Eva S Bayu, 4 Trimurni Abidin, 5 Syafrudin Ilyas 6 Marline Nainggolan, 7 Endang Syamsudin ABSTRACT Aim: The aim of this study is to compare whether there are differences in the value content of secondary metabolites (saponins, tannins, and favonoids) between the group treated with Binahong leaves (administration of colchicines) and the group treated with Binahong leaves without the application of colchicines. Materials and methods: The design of this study is an experimental research laboratory study. This research was done in four different places in which colchicines were applied to improve the quality of Binahong, conducted in the USU Agricultural Faculty’s Greenhouse, whereas no application of colchicines was done in the Desa Simpang Pergendangan Kelurahan Tiga Binanga Kabupaten Karo harvesting Binahong plant. The pharmacy faculty of USU was chosen as a place to obtain the extract and the Laboratory of the Research Institute for Spices and Medicinal Plants (Balitro) was where the phyto- chemical analysis was conducted. Results: The results showed that there were differences in the descriptive values of secondary metabolites in the Binahong leaf extract, such as saponins, tannins, and favonoids, wherein the group with colchicines was higher than groups without colchicines, but there was no statistical differences between the groups treated with colchicines and those not treated with colchicines (value p > 0.05). ORIGINAL RESEARCH 1 Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Sumatera Utara, Medan, Indonesia 2,3 Agroecotechnology Studies Program, Faculty of Agriculture University of Sumatera Utara, Medan, Indonesia 4 Department of Conservative Dentistry, Faculty of Dentistry University of Sumatera Utara, Medan, Indonesia 5 Department of Biology, Faculty of Mathematics and Science University of Sumatera Utara, Medan, Indonesia 6 Department of Pharmacology, Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara Medan, Indonesia 7 Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Padjajaran, Indonesia Corresponding Author: Olivia A Hanafiah, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Sumatera Utara, Medan, Indonesia, e-mail: olivia_avriyanti@ yahoo.com 10.5005/jp-journals-10015-1453 Conclusion: Administration of colchicines can improve the content of secondary metabolites in Binahong leaf extract and the plant morphology without affecting the content of the plant extract. Clinical signifcance: Secondary metabolites in herbal plants, such as saponins, favonoids, and tannins are able to speed up the healing process. One of the plants that have all three of these active compounds is Binahong (Anredera cordifolia). Binahong leaf extract can stimulate the process of fbroblasts and collagen formation that will accelerate the process of wound healing. Colchicine as a reagent for the mutation is able to affect the number of leaves and morphological characteristics of the Binahong plant. Keywords: Binahong leaf extract, Colchicine, Secondary metabolites, Wound healing. How to cite this article: Hanafah OA, Hanafah DS, Bayu ES, Abidin T, Ilyas S, Nainggolan M, Syamsudin E. Quantity Dif- ferences of Secondary Metabolites (Saponins, Tannins, and Flavonoids) from Binahong Plant Extract (Anredera Cordifolia (Ten.) Steenis) treated and untreated with Colchicines that play a Role in Wound Healing. World J Dent 2017;8(4):296-299. Source of support: The research was funded by Direktorat Riset dan Pengabdian Masyarakat Direktorat Jenderal Pengua- tan Riset dan Pengembangan Kementerian Riset, Teknologi, dan Pendidikan Tinggi, sesuai dengan Surat Perjanjian Penu- gasan Pelaksanaan Program Penelitian, nomor: 017/SP2H/LT/ DRPM/2/2016, tanggal 17 February 2016. Confict of interest: None INTRODUCTION Anredera cordifolia (Ten.) steenis also known as Binahong is a family of Basellaceae, which is a medicinal plant that has been growing very well since long. In Indonesia, the Binahong plant is still uncommon but in Vietnam, this plant has a high demand and is often used as a vegetable in Taiwan. In China and Taiwan, this plant is known to have tremendous benefits and has been consumed more than a thousand years ago. Almost all parts of the Bina- hong plant, such as tubers, stems, and leaves can be used in herbal therapy. 1 Binahong leaf extract can stimulate fibroblasts and collagen formation, which accelerates the process of wound healing. 2,3 Optimal Binahong growth and productivity are determined by the type of plant and the contents of the secondary metabolites. A little genetic variation may