Hybrid Nano Plasmonics for Integrated Biosensor Chii-Wann Lin *a , Jun-Haw Lee b , Nan-Fu Chiu a , Szu-Yuan Lee a , Kou-Chen Liu c , Feng-Yu Tsai d , Chia-Yu Yen e , Chun-Nan Lee f *a Inst. of Biomed. Eng., b Inst. of Optcal Eng., d Inst, of Mat. Sci and Eng., e Dept. of Mech. Eng., f Dept. of Clin. Lab. Sci. Med. Biotech., National Taiwan Univ., No.1, Sec.4, Roosevelt Rd., Taipei, Taiwan 106; c Inst. of Opt. eng., Chang-Gang Univ., ABSTRACT SPR biosensor with OLED and nano-grating for HBV LAMP product detection is reported. Directional emissions by grating-coupler match the resonant condition of SP modes. Concentration changes result in color shift at specific angle. Real time detection of virus load down to 5 copies/25 ul can be achieved in 30 minutes. Surface plasmon Resonant (SPR) biosensor has been used for quantitative measurement of molecular interactions for its advantages of high sensitivity, label-free and real-time detection. In this paper, we report recent efforts on further enhancement of SPR biosensors by the heterogeneous integration of organic electroluminescence light source and nano-grating structure for the feasibility study on the fast and high sensitivity detection of HBV isothermal amplification products, Mg 2 P 2 O 7 . We demonstrated the surface plasmon coupled through hybrid nano-grating structure has highly directional emissions corresponding to the resonant condition of surface plasmon modes on the Au/air interface and controllable plasmonics band-gap by pitch modulation. SPGCE resulted in color change from yellowish green to orange at a certain viewing angle, when contacting glucose with concentration increasing from 10 to 40%. Keywords: (170.3890) Medical optics instrumentation; (240.6680) Surface plasmons 1. INTRODUCTION Advancements in biotechnology and micro/nano-technologies have resulted in the fast evolution of biosensors and biochips for applications in both diagnosis and therapy, for examples drug discovery and disease marker profiling based on molecular recognition 1 . One of the most widespread label-free optical detection systems is surface-plasmon resonance (SPR). The behavior of free electrons or plasma near a metallic-dielectric interface is a special type of light-matter interactions. High-density electron gas, when subjected to collective longitudinal excitation or oscillation, will manifest particle-like behavior. Analogous to phonons, magnons and excitons, such a phenomenon has been called “Plasmon”. It occurs in the forms of bulk, surface, and particle plasmons, and research on the phenomenon has led to many important applications in optoelectronics and biomedicine. The unique characteristics of non-labeling and real-time monitoring have opened up a wide spectrum of novel developments in biochemical and biophysical applications, especially those involving the dynamic interactions of bio-molecules at the solid/liquid or solid/gas interface. The most prevalent implementation for product development is based on the prism coupling of Attenuate Total Reflection (ATR). With this method, biochemical interactions on the sensor surface are monitored by observing the resonant behavior of surface plasmon waves on the metal/dielectric interface. However, many of these systems require the uses of bulky high refractive index coupling prism, external light source and detector to measure the resonant condition by reflectivity. Currently, we are developing an integrated plasmonic device for a hand-held SPR system. We have developed three components of such a system, including, multi-layer 2,3 , nano-grating 4,5 and active-plasmonics 6 for further minimization of the whole system onto a disposable platform with controllable planar electroluminescence light source through modulation of nanostructure as shown in Fig 1. Fig. 1. Schematic diagram of the proposed hybrid nano-plasmonic sensor for biomolecular interactions. Invited Paper Optical Sensors and Biophotonics, edited by Xingde Li, Qingming Luo, Vasilis Ntziachristos, Yoshiaki Yasuno, Proc. of SPIE-OSA-IEEE Asia Communications and Photonics, SPIE Vol. 7634, 76340O · © 2009 SPIE-OSA-IEEE CCC code: 0277-786X/09/$18 · doi: 10.1117/12.853214 SPIE-OSA-IEEE/ Vol. 7634 76340O-1