Int.J.Curr.Microbiol.App.Sci (2019) 8(9): 1346-1351 1346 Original Research Article https://doi.org/10.20546/ijcmas.2019.809.154 Investigation of Sero Prevalence of Brucella Outbreak in an Organised Dairy Farm Sipra Panda 1 *, Mayuri Chelkar 1 , S. P. Chaudhari 1 , N. V. Kurkure 1 and S. W. Kolte 2 1 Department of Veterinary Pathology, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Nagpur - 440006, India 2 Department of Veterinary Parasitology, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Nagpur, Maharashtra – 440006, India *Corresponding author ABSTRACT Introduction Brucellosis is a potential zoonotic disease having world-wide distribution. It is caused by a Gram negative bacteria Brucella abortus in cattle, B. melitensis in goats and sheep and B. suisin swine characterised by abortions, infertility and reduced milk yield (Mantur et al., 2007). Bovine brucellosis is an endemic disease in most part of the states in India (Islam et al., 2013) and the trend appears to be on the increase in recent times, perhaps due to trading and movement of livestock within the states. Brucella infection in endemic areas requires screening and rapid diagnosis to avoid high economic loss, morbidity. This may cause havoc to mankind if not kept under check (Atluri et al., 2011). The serological assays which are mostly performed in farm International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 09 (2019) Journal homepage: http://www.ijcmas.com Brucellosis is a zoonotic disease with epidemiological global effect. It has been found to affect the cattle industry worldwide and India is not an exception to it. The current sero survey was carried to illuminate the status of bovine brucellosis in an organised dairy farm from Nasik with past history of abortions. Total of 65 serum samples were collected from cattle and tested for the presence of brucella antibodies using Rose Bengal Plate Test (RBPT), Standard Tube Agglutination Test (STAT) and Indirect Enzyme Linked Immnuno-Sorbent assay (I-ELISA). Out of 65 samples, 25 (38.4%) sera were positive by RBPT, and 19(29.2%) for STAT. Screening by Indirect ELISA revealed that 26 samples (40.0%) were positive for brucellosis. Overall, seroprevalence in the herd was found to be 23 (35.3%). The present study highlights the importance of study of seroprevalence in cattle population to eliminate the economic loss among farmers and raise awareness to avoid zoonoses caused by brucella infection. Keywords Brucellosis, ELISA, abortion, zoonoses, seroprevalence Accepted: 15 August 2019 Available Online: 10 September 2019 Article Info