_____________________________________________________________________________________________________ *Corresponding author: Email: aminsaupp@yahoo.com; Asian Food Science Journal 19(3): 25-38, 2020; Article no.AFSJ.62668 ISSN: 2581-7752 Impact of Different Substrates and Mother Cultures on Yield and Yield Attributes of Oyster Mushroom (Pleurotus ostreatus) M. Mubasshira 1 , F. M. Aminuzzaman 1* , N. Sultana 1 and J. F. Tanni 1 1 Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e- Bangla Nagar, Dhaka-1207, Bangladesh. Authors’ contributions This work was carried out in collaboration among all authors. Author MM performed the experiment. Author JFT prepared the manuscript. Author FMA conceived, designed, supervised the study and corrected and edited the manuscript. Author NS managed the literature searches. All authors read and approved the final manuscript. Article Information DOI:10.9734/AFSJ/2020/v19i330241 Editor(s): (1) Dr. Surapong Pinitglang, University of the Thai Chamber of Commerce, Thailand. Reviewers: (1) M. K. Khokhar, ICAR - National Centre for Integrated Pest Management, India. (2) Parveez Ahmad Sheikh, Sher-e-Kashmir University of Agricultural Science & Technology- Kashmir, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/62668 Received 29 September 2020 Accepted 04 December 2020 Published 16 December 2020 ABSTRACT Aim: This study was aimed to compare the performance of different substrates and mother culture materials on yield and yield parameters of oyster mushroom (Pleurotus ostreatus). Study Design: This is an experimental study following Complete Randomized Design (CRD). Place and Duration of Study: The experiments were conducted in the Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka and Mushroom Development Institute, Savar, Dhaka from January 2019 to February 2020. Methodology: Accordingly, three substrates (sawdust, rice straw, sawdust + rice straw (1:1)) and three mother cultures (rice, maize, sawdust) were used in oyster mushroom cultivation. Results: Among the substrates and mother culture components, using rice straw and sawdust mother spawn, the maximum length of stipe was recorded (23.27 mm and 24.29 mm, respectively). Applying sawdust + rice straw (1:1) and maize mother spawn, the peak diameter of stipe was calculated (9.90 mm and 10.01 mm, respectively). The maximal diameter of pileus was observed in sawdust + rice straw (1:1) and rice mother spawn (72.90 mm and 67.57mm, respectively). With the Original Research Article