ANNOTATED SEQUENCE RECORD Full-genome sequence of a novel myovirus, GF-2, infecting Edwardsiella tarda: comparison with other Edwardsiella myoviral genomes Motoshige Yasuike 1 • Issei Nishiki 1 • Yuki Iwasaki 1 • Yoji Nakamura 1 • Atushi Fujiwara 1 • Emi Sugaya 2 • Yasuhiko Kawato 3 • Satoshi Nagai 1 • Takanori Kobayashi 1 • Mitsuru Ototake 1 • Toshihiro Nakai 4 Received: 27 January 2015 / Accepted: 27 May 2015 Ó Springer-Verlag Wien 2015 Abstract Edwardsiellosis, which is caused by Edward- siella tarda, a Gram-negative bacterium, is one of the most serious infectious diseases in both marine and freshwater fish farms worldwide. Previously, we reported the complete genome sequences of three E. tarda-lytic bacteriophages (two podoviruses and a myovirus), which were isolated from fish tissues and fish-rearing seawater. Further geno- mic information regarding E. tarda phages is important for understanding phage-host interactions as well as for applications of the phages for the control of disease. Here, we report the complete genome sequence of a novel E. tarda phage (GF-2) of myovirus morphology (family My- oviridae), isolated from tissue homogenates of a cultured Japanese flounder (Paralichthys olivaceus) that succumbed to edwardsiellosis in Japan. The size of the entire genome was 43,129 bp, with a GC content of 51.3 % and con- taining 82 open reading frames (ORFs). The GF-2 genome possesses lysogeny-related genes that have not been found in the reported Edwardsiella phage genomes. Comparative genomics of Edwardsiella myophages suggest that the C-terminal domains of the tail fiber proteins have relevance to their host specificity. Thus, GF-2 genome information provides a novel resource for our understanding of the molecular mechanisms involved in their host specificity and for detection of E. tarda in aquaculture environments. Abbreviations RAST Rapid annotations using subsystems technology BLAST Basic local alignment search tool ORF Open reading frame nt Nucleotide aa Amino acid DDBJ DNA data bank of Japan Edwardsiella tarda, a member of the family Enterobacte- riaceae, is a pathogen of a wide variety of farmed fish as well as other animals and humans. E. tarda infection (ed- wardsiellosis) is one of the most serious economic threats to marine and freshwater aquaculture in the world [re- viewed in reference 5]. The intracellular parasitic nature of E. tarda makes chemotherapeutic treatment and develop- ment of vaccines for the disease difficult. The bacterium isolated from most infected fish species is motile by per- itrichous flagella and is designated as the typical strain, while those isolated from marine fish such as red sea bream (Pagrus major), crimson sea bream (Paragyrops edita) or yellowtail (Seriola quinqueradiata) are non-motile Electronic supplementary material The online version of this article (doi:10.1007/s00705-015-2472-5) contains supplementary material, which is available to authorized users. & Atushi Fujiwara jiwara@affrc.go.jp Motoshige Yasuike yasuike@affrc.go.jp 1 Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, Japan 2 Department of Marine Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Hiroshima, Japan 3 Diagnosis and Training Center for Fish Diseases, National Research Institute of Aquaculture, Fisheries Research Agency, Minami-Ise, Mie, Japan 4 Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan 123 Arch Virol DOI 10.1007/s00705-015-2472-5