Hamad et al (2020): Effects of T gondii strains on female with POS September 2020 Vol. 23 Issue 13B 6295/ASRO.2020.231362 http://doi.org/10.3 Health Annals of Tropical Medicine & Public Effects of Two Genotypes of Toxoplasma gondii Strains on DNA Sequence of Females’ Oocytes with Polycystic Ovarian Syndrome Dr. Sadiashahab Hamad 1 , Batool A.H. Al-Haidary 2 , Dr. Zaman Abdul-Sahib Abed 3 1. College of Science, University of Kirkuk, Iraq. 2. College of Health & Medical Technology/ Middle Technical University, Iraq. 3. College of Nursing, University of Baghdad, Iraq. *Corresponding author: Sadiashahab Hamad (asst.prof.dr.sadia@gmail.com ) Abstract Polycystic ovary syndrome (PCOS) is one of the conditions that affects married and unmarried women and causes problems in childbirth. A part of extracellular matrix in the ovary is the glycoprotein known as CYP1A1 gene. Twenty blood specimens were collected from female with PCOS suffering from toxoplasmosis. .In addition to 20 blood samplesfor women infected with Toxoplasmosis as diseasecontrol group, the results of investigation were compared with those of the same number of blood samples from age-matched apparently healthy women as a control group. Whole blood specimens were used to carry out a (PCR), while ELISA used to measure Anti- Toxoplasma IgG antibody levels in the sera of the above studied groups. The results showed that there was no significant variation between patients different age groupsin comparison withboth control groups (P>0.05), also there was no significant difference between rural and urban patients (P>0.05).Additionally, no significant differences were between single women 11(55%), and married 9(45%) compared to Toxoplasma control 10(50%) and healthy control 10(50%) respectively (P>0.05). The findings showed that there was highly mutation in CYP1A1 gene of polycystic ovary syndrome (PCOS) with toxoplasmosis that were 10(50%) out of 20 (100%) of cases, but there was no significant difference among age group, residency, marital status and mutation with Toxoplasma infection and PCOS cases (P>0.05). Clonal detection of T. gondii by PCR technique identified 10(50%) cases of type II and 10(50%) cases of type I for all categories in the study, twenty were taken to complete the reaction phase within the thermal polymer and were deported in to the reaction solution electrically (2.5%) agarose gel with the use the volumetric guide DNA ladder (M) 100 bp size. In 8 PCOS patients, an experiential evaluation was seen at G to A conversion in nucleotide 6325. This synonymous substitution (SNP G6325A) took place in the 5 th positions of prolong codonleading to change the codon TGC to TAC. Key words: Toxoplasma gondii, Genotype, PCOS, oocyte, CYP1A1 gene, mutation How to cite this article: Hamad S, Al-haidary BAH, Abdul-Sahib Abed Z (2020): Effects of two genotypes of Toxoplasma gondii strains on DNA sequence of females’ oocytes with polycystic ovarian syndrome, Ann Trop Med & Public Health; 23(S13B): SP231362. DOI: http://doi.org/10.36295/ASRO.2020.231362 Introduction Toxoplasmosis is one of the most serious complications of both men and women, andleads to cancer, abortion and also infertility for both sexes [1]. Polycystic ovary syndrome is a common condition in women with young and old ages that leads to non-reproduction [2].Apart of extracellular matrix in the ovary is the glycoprotein now as CYP1A1 gene[3]. Several studies have shown that toxoplasmosis is a cause of cancer, such as breast cancer and uterine cancer [4]. The incursion of this parasite to different