677 Racial Differences In The Relationship Of Total and Food- Specific IgE To Atopic Dermatitis In Childhood Dr. Gillian Bassirpour, MD 1 , Dr. Edward M. Zoratti, MD, FAAAAI 2 , Ganesa Wegienka, PhD 3 , Ms. Suzanne Havstad, M.A. 3 , Alexandra Sitarik, MS 3 , Dr. Haejin Kim, MD 1 , Dr. Dennis Ownby, MD, FAAAAI 4 , Dr. Christine Cole Johnson, PhD, MPH, FAAAAI 3 ; 1 Division of Allergy and Clinical Immunology, Henry Ford Hospital, Detroit, MI, 2 Henry Ford Health System, Detroit, MI, 3 Department of Public Health Sciences, Henry Ford Hospital, Detroit, MI, 4 Department of Pediatrics Georgia Re- gents University, Augusta, GA. RATIONALE: Elevated IgE is a hallmark of atopic dermatitis (AD). Although AD and elevated IgE are more prevalent in African American (AA) compared to Caucasian (C) children, it is unknown if the relationship between AD and IgE is similar between these groups. METHODS: Data were analyzed from 371 AA and 138 C children in the Detroit WHEALS birth cohort. AD was defined as current or past physician diagnosis as determined at a study-related encounter at 2-3 years of age. Total and food-specific IgE (sIgE) levels for peanut, egg, and milk were determined. Univariate and multiple logistic regression models, stratified by race of child, were used to assess the association of AD with both total IgE and food sensitization. Food sensitization was included in the model as either the sum of the 3 food sIgEs or, separately, having at least one positive sIgE (> _0.35 kU/L) to food. RESULTS: Elevated total IgE was independently associated with AD only among Caucasian children [adjusted odds ratio (aOR) 1.81, 95% CI 1.03, 3.15, p 5 0.038]. In contrast, the sum of the sIgEs for the foods [aOR 1.46, 95% CI 1.15, 1.86, p 5 0.002] or the presence of > _ 1 positive food sIgE [aOR 2.05, 95% CI 1.09, 3.87, p 5 0.027] was independently associated with AD among AA children only. CONCLUSIONS: The association of IgE to AD differs among AA and C children. Food sIgE is independently linked to AD among AA children. Such an association is not evident in C children after adjustment for total IgE. 678 Major Culprit Allergen Sensitization Patterns According To Age In Korean Atopic Dermatitis Patients Hye Jung Park 1 , Jae-Hyun Lee 1,2 , Kyung-Yong Jeong 2 , Kyung-Hee Park 1,2 , Yoon-Ju Kim 2 , Jung-Won Park 1,2 ; 1 Division of Allergy and Clin- ical Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea, 2 Institute of Allergy, Yonsei University College of Medicine, Seoul, South Korea. RATIONALE: Atopic dermatitis (AD) usually occur in childhood and some may be remitted but others persist to adulthood. Culprit allergens may be changed as the disease progress. In this study, we tried to distinguish important culprit allergens according to age in AD patients. METHODS: Total 110 AD patients’ sera were tested to detect allergen specific immunoglobulin E (sIgE) for each 11 allergens - milk, egg white, peanut, shrimp, wheat, Dermatophagoides farinae (DF), Candida albicans (CA), Trichophyton rubrum (TR), Pityrosporum ovale(PO), Staphylococcal enterotoxin B (SBE), recombinant Hom s 1. Mean age of the enrolled patients was 12.2 years olds (range: 1;47). RESULTS: Sensitization rates of allergens were varied from 17.3% (for TR) to 52.7% (for DF). Milk and egg white sIgEs were more detected in young aged patients and their sensitization rates decreased according to age increment. Conversely, sensitization rates of shrimp, DF, SBE, CA, TR, PO and SBE increased according to age increment. There were no differences in peanut and wheat sensitization rates. Interestingly, rHom s 1 sIgE was detected more frequently in younger patients (36.5% in age 1-5 vs. 12.1% in age > _ 18). CONCLUSIONS: There was distinct difference of sensitization pattern. Milk and egg white are more important allergens in young aged patients. On the contrary, microbial and house dust mite allergens were more important in adult patients. Autoantigen can also be important cause at early stage of AD. 679 Protein Microarray: IgE-Profiling Of Brazilians With Atopic Dermatitis Lucila Camargo Lopes de Oliveira 1 , Roberta Faria Camilo-Ara ujo 1 , Isa- bel Rugue Genov 1 , Dr. Renata R. Cocco 2 , Dr. Marcia Mallozi, MD 3 , Prof. Nelson A. Rosario, MD, PhD, FAAAAI 4 , Prof. Dirceu Sole, MD, PhD 5 ; 1 UNIFESP, 2 Federal University of S~ ao Paulo, 3 Federal University of S~ ao Paulo, Brazil, 4 Federal University of Parana, Curitiba, Brazil, 5 Fed- eral University of Sao Paulo, Sao Paulo, Brazil. RATIONALE: to identify for the first time the IgE-sensitization profile among Brazilian patients with different severity of atopic dermatitis (AD) using protein microarray techniques. We hypothesized the greater the severity the disease, the more common the sensitization to food allergens. METHODS: 59 AD patients classified according to the severity scoring of AD index (SCORAD,SI) took part on this study. Peripheral blood samples were collected after consent approval and their IgE-sensitizing profile was determined using ImmunoCAP-ISAC TM (Thermo Scientific). The study was approved by the Brazilian ethical committee (# 12551413.2.0000.5505 on www.saude.gov.br/plataformabrasil, funded by FAPESP n o 2009/53303-3). Data were expressed as mean, median and range. Kruskal-Wallis test was used to analyze differences among groups since they had no normal distribution. RESULTS: patients aged from 0.75 to 23.4 years (mean 5 8.25y) were distributed as following: mild (A, n517, median age 5 12.2y, mean SI 5 23.7), moderate (B, n513, median age 5 8.5y, mean SI 5 42.25), and severe (C, n529, median age 58.0y, mean SI 5 54.64). Mean sensitization (number of allergens per individual) to all allergens [A:8,6 (range 2-38), B:14,7 (6-41), C:13,3 (1-39)] and just to food allergens [A:1,7 (0-18); B:3,8 (0-21); 3,4 (0-19)] were not significant different between groups (Kruskal-Wallis test, p50.0641). CONCLUSIONS: food sensitization was not related to intensity of atopic dermatitis on this Brazilian studied group. 680 Relationship Between Dietary Food and Nutrient Intakes and Bone Mineral Density In Childhood Eczema Dr. Ting Fan Leung, MD, FRCPCH, FAAAAI, Ms. Flora Yin-ying Kwok, MPhil, Dr. Yvonne Yi-fong Ho, MPhil, Dr. Susan Shuxin Wang, PhD, Ms. Patty Pui-pui Tse, MSc, Prof. Gary Wing-kin Wong, MD, FRCPC, Dr. Kam Lun Ellis Hon, MD, FAAP; Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong. RATIONALE: Food avoidance is common among eczema children, but there is limited data regarding effects of such practice on bone mineral density (BMD). This study evaluated nutrient intakes and BMD in Hong Kong eczema children. METHODS: Chinese children with and without eczema were recruited from allergy or dermatology clinics. Their nutrient intakes were assessed by food frequency questionnaire and analyzed using Foodworks Professional software (Xyris, Brisbane, Australia). BMD was measured at mid-point of radius in the non-dominant arm and left tibia by quantitative ultrasound bone sonometry (Omnisense 7000P, BeamMed, Tikva, Israel). RESULTS: 114 eczema children and 60 controls were recruited. Calcium and vitamin D intakes were lower in moderate-to-severe eczema (50) and mild (64) eczema than controls (medians for calcium in mg/MJ: 68.1, 74.4 and 89.5, P50.001 for trend; for vitamin D in mg/MJ: 0.10, 0.14 and 0.27, P50.001 for trend). The mean (SD) BMD z-scores in eczema and controls were 0.52 (0.90) and 0.55 (1.12) at radius (P50.889) and 0.02 (1.03) and -0.03 (1.13) at tibia (P50.886). Among eczema children, BMD at radius was higher in those with first and third tertiles of calcium intake than those in the second tertile (P50.016). BMD at tibia correlated positively with calcium intakes (P50.015). CONCLUSIONS: This study characterizes pattern of dietary restriction in Hong Kong eczema children, who have low calcium and vitamin D intakes. Despite this, BMD is similar between eczema and controls. Higher BMD is found in patients with higher calcium intakes. Funding: Research Committee Group Research Scheme (3110087) and Direct Grant for Research (2011.1.058), CUHK J ALLERGY CLIN IMMUNOL FEBRUARY 2014 AB196 Abstracts MONDAY