Biosaintifika 9 (1) (2017) 148-155 Biosaintifika Journal of Biology & Biology Education http://journal.unnes.ac.id/nju/index.php/biosaintifika Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Cancer Cells  Nuraeni Ekowati, Aris Mumpuni, Juni Safitri Muljowati DOI: 10.15294/biosaintifika.v9i1.7546 Faculty of Biology, University of Jenderal Soedirman, Purwokerto, Indonesia Abstract showed that the application of bioactive compounds 100 µg / ml resulted in an increase of in death of HeLa cells along with length of incubation time. An impor- tant finding was that HeLa cells death by apoptosis was greater than by necrosis. In conclusion, the extracts of P. ostreatus has the potential to inhibit the growth of HeLa cells. How to Cite Ekowati, N., Mumpuni, A. & Muljowati , J. S. (2017). Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Cancer Cells. Biosaintifika: Journal of Biology & Biology Education, 9(1), 148-155. © 2017 Universitas Negeri Semarang History Article Received 23 October 2016 Approved 14 February 2017 Published 1 April 2017 Keywords apoptosis; bioactive com- pounds; cytotoxic test; HeLa cell; Pleurotus ostreatus  Correspondence Author: Jl Dr. Soeparno No. 63, Grendeng, Purwokerto, Jawa Tengah 53122 E-mail: nuraeniekowati@yahoo.com p-ISSN 2085-191X e-ISSN 2338-7610 Pleurotus ostreatus is a common mushroom cultivated in Indonesia, ZLWK potential properties of bioactive compounds for medicinal mushroom. This study was aimed at obtaining P.ostreatus extract bioactive compounds potential in inhibiting the pro- liferation of cervical cancer cells (HeLa) and evaluating the HeLa cell proliferation kinetics and HeLa cell death mechanisms. The research was beneficial in making this product can be easily applied in a more controlled industrial scale. Antican- cer activity test through a cytotoxic test using the MTT [3- (4,5-dimetiltiazol-2-yl) -2.5-diphenyl tertrazolium bromide], the kinetics proliferation of HeLa cells and HeLa cell death mechanism wHUH performed.Linear regression analysis was used to analyze the data. Ethyl acetate extract of P. ostreatus isolated from Madiun showed the best results with IC 50 = 107.59 µg / ml. HeLa cell proliferation kinetics analysis