Advances in Breast Cancer Research, 2018, 7, 91-106 http://www.scirp.org/journal/abcr ISSN Online: 2168-1597 ISSN Print: 2168-1589 DOI: 10.4236/abcr.2018.72006 Mar. 15, 2018 91 Advances in Breast Cancer Research Detection of Promoter Hypermethylation of GSTP1 and CDH1 Genes and the Relationship of Histopathological Parameters of the Breast Onur Eroglu 1,2* , Mine Erci Baysak 3 , Beyhan Durak Aras 3 , Oguz Cilingir 3 , Sevilhan Artan 3 1 Department of Molecular Biology and Genetics, Faculty of Art and Science, Bilecik Seyh Edebali University, Bilecik, Turkey 2 Biotechnology Research and Application Center, Bilecik Seyh Edebali University, Bilecik, Turkey 3 Department of Medical Genetics, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey Abstract Background: Breast cancer is the most common cancer in women. Histopa- thology plays an important part in determining the treatment strategy for women with breast cancer. GSTP1 plays an important role in protecting cells from cytotoxic and carcinogenic agents and it is expressed in normal tissues at variable levels in different cell types. CDH1 plays a critical role for establish- ment and maintenance of polarity and differentiation of epithelium during the development period. Also, it plays an important role in signal transduc- tion, differentiation, gene expression, cellmotility and inflammations. Me- thods: In this study the promoter methylation levels of GSTP1 and CDH1 gene which are associated with breast cancer were investigated by technique of Methylation Sensitive High Resolution Melting Analysis (MS-HRM). We analysed primary tumor core biopsies from 80 high-risk primary breast cancer patients (tumors ≥ 2 cm and/or lymphatic metastase and/or distant metastases and/or under 40 years). Also the patients’ histopathologic types were asso- ciated with the methylation levels. Results: In our study the promoter hyper- methylation status was observed at different rates; GSTP1 and CDH1 hyper- methylation frequencies were 82% and 95% respectively. The promoter hypermethylation levels of the genes were found to be significant with lymph node positivity, ER positivity and HER2/neu negativity. Conclusion: Our study is important as being the first study that analyzes association between histopathologic type and GSTP1 and CDH1 gene promotor methylation status in Turkish population. Keywords Breast Cancer, Methylation, GSTP1, CDH1, Histopathological Types How to cite this paper: Eroglu, O., Baysak, M.E., Aras, B.D., Cilingir, O. and Artan, S. (2018) Detection of Promoter Hyperme- thylation of GSTP1 and CDH1 Genes and the Relationship of Histopathological Pa- rameters of the Breast. Advances in Breast Cancer Research, 7, 91-106. https://doi.org/10.4236/abcr.2018.72006 Received: December 23, 2014 Accepted: March 12, 2018 Published: March 15, 2018 Copyright © 2018 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access