Research Article A Validated Method for the Quantitation of Ciprofloxacin Hydrochloride Using Diffuse Reflectance Infrared Fourier Transform Spectroscopy Bhoomendra Bhongade, Sirajunisa Talath, and Sunil Dhaneshwar Department of Pharmaceutical Chemistry, RAK College of Pharmaceutical Sciences, RAK Medical & Health Sciences University, P.O. Box 11172, Ras Al Khaimah, UAE Correspondence should be addressed to Bhoomendra Bhongade; ba.bhongade@gmail.com Received 31 October 2013; Accepted 22 December 2013; Published 5 February 2014 Academic Editor: Hakan Arslan Copyright © 2014 Bhoomendra Bhongade et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A quantitative method using difuse refectance infrared Fourier transform spectroscopy (DRIFTS) was developed and validated for the estimation of ciprofoxacin in its tablet dosage forms. Te solid-state samples were prepared by dilution in dry potassium bromide and were analyzed by FTIR spectrophotometer with DRIFT sampling technique. A linear relationship for the carbonyl peak area centered around 1709 cm -1 was observed in the range of 0.3–1.5% w/w with good correlation coefcient of 0.998. Te percent recovery of ciprofoxacin in three marketed tablet dosage forms was in the range of 98.76 ± 0.27. Te present reported method is precise, reproducible, and eco-friendly. DRIFTS may have a potential as an alternative method for qualitative and quantitative analysis of ciprofoxacin in bulk drugs and tablet dosage forms. 1. Introduction Fourier transform infrared spectroscopy (FTIRS) is a widely explored technique in the pharmaceuticals and drug research for the identifcation of compounds, impurities, and deter- mination of functional groups in qualitative analysis. Tra- ditionally, FTIR analysis is carried out by transmission measurement technique using the transparent pallets of sample with halide salts. Quantifcation of some pharma- ceutical agents has been reported in the literature using FTIR spectroscopy either by measuring the transmis- sion of analyte in potassium bromide or in chloroform [1–4]. Because of the availability of newer sampling techniques in handling samples, FTIR is gaining more attention of the analytical researchers in the exploration and use of FTIRS technique for the quantitative analysis of solid-state samples. Difuse refectance infrared fourier transform (DRIFT) was proposed to be relatively better techniques for quantita- tive analysis of solid-state samples while attenuated total refectance (ATR) sampling technique is more preferred method for qualitative analysis [5]. Te practical difculty that may arise for the quantifcation of solid-state samples using ATR and transmission measurement may be the repro- ducibility which can be minimized using difuse refectance sampling technique. Te technique of DRIFT spectroscopy is concerned with the efcient collection of difusely scattered light at the direction unrelated to that of the incident radiation. Te Kubelka-Munk model described the theory of difuse refectance at scattering surfaces which relates band intensities to concentration for transmission measurements similar to Beer’s law [6]. Te literature revealed the use of DRIFTS for quantitative estimation of mixtures of sulfame- thoxazole polymorphs [7], simultaneous quantitation of ethenzamide, isopropylantipyrine, cafeine, and allyliso- propylacetylurea in tablet [8]. Tus, DRIFTS may have the potential to provide an efcient method for the solid-state quantifcation of pharmaceuticals. Te fuoroquinolones represent one of the major syn- thetic classes of antibacterial agents with bactericidal activity Hindawi Publishing Corporation International Journal of Spectroscopy Volume 2014, Article ID 294612, 6 pages http://dx.doi.org/10.1155/2014/294612