S. Aravind Kumar, Ramachandra Prabhakar, N. Raj Vikram, SP Saravana Dinesh, S Rajeshkumar. Antioxidant Activity of Silymarin/Hydroxyapatite/Chitosan Nano Composites - An In Vitro Study. Int J Dentistry Oral Sci. 2021;8(1):1402-1404. 1402 OPEN ACCESS https://scidoc.org/IJDOS.php Antioxidant Activity of Silymarin/Hydroxyapatite/Chitosan Nano Composites - An In Vitro Study Research Article S. Aravind Kumar 1 , Ramachandra Prabhakar 2 , N. Raj Vikram 3 , SP Saravana Dinesh 4 , S Rajeshkumar 5* 1 Research Scholar, Department of Orthodontics, Dr. MGR Educational and Research Institute, Chennai, India. 2 Dean, Professor & Head, Department of Orthodontics, Thai Moogambigai Dental College and Hospital, Chennai, India. 3 Professor, Department of Orthodontics, Thai Moogambigai Dental College and Hospital, Chennai, India. 4 Professor, Department of Orthodontics, Saveetha Dental College and Hospital SIMATS, Saveetha University, Chennai, India. 5 Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental College and Hospital, SIMATS, Saveetha University, Chennai, India. International Journal of Dentistry and Oral Science (IJDOS) ISSN: 2377-8075 *Corresponding Author: Dr. S Rajesh Kumar, Associate Professor, Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental College and Hospital, SIMATS, Saveetha University, Chennai, India. E-mail: ssrajeshkumar@hotmail.com Received: January 12, 2021 Accepted: January 22, 2021 Published: January 26, 2021 Citation: S. Aravind Kumar, Ramachandra Prabhakar, N. Raj Vikram, SP Saravana Dinesh, S Rajeshkumar. Antioxidant Activity of Silymarin/Hydroxyapatite/Chitosan Nano Composites - An In Vitro Study. Int J Dentistry Oral Sci. 2021;8(1):1402-1404. doi: http://dx.doi.org/10.19070/2377-8075-21000278 Copyright: S Rajeshkumar © 2021. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribu- tion and reproduction in any medium, provided the original author and source are credited. Introduction The oxygen utilization characteristic in cell development leads to the age of a progression of receptive oxygen species (ROS). ROS include free radicals such as superoxide anion radicals (O 2 ), 2 hy- droxyl radicals (OH•) and non free-radical species such as hydro- gen peroxide (H 2 O 2 ) and single oxygen [1-3]. ROS at physiological concentration might be needed for typical cell work. They are likewise equipped for damaging critical bio- molecules, for example, nucleic acids, lipids, proteins, polyunsatu- rated unsaturated fats and carbs and may cause DNA damage that can prompt changes [4, 5]. Silymarin, a known standardized extract obtained from seeds of Silybummarianum (Milk Thistle ) is widely used in treatment of several diseases. Silymarin, a plant derived favonoid, which is named benzopyranone, is secluded from the products of the soil of the milk thorn (Silymarinmarianum) is a combination of three primary parts: silibinin, silydianine and silychristine. It has been known for its hepatoprotective antibacterial, antiallergic, anti- mutagenic, antiviral, hostile to neoplastic, antithrombotic special- ists and vasodilatory activities. One of the signifcant issues with respect to silymarin is that it could be acknowledged as a natural compound [6-9]. Hydroxyapatite (HA )possess signifcant mechanical strength, biocompatibility, low resorbability, osteoconductivity, HA is ex- tensively used in orthopedic and dentistry as metallic implant coating and cavity flling material and also, as the key constituent in toothpaste and mouth washes to remineralize artifcial carious lesions [10]. Chitin, especially its deacetylated form, and chitosan are well known marine biopolymers, having many applications in the food industry, agriculture, biotechnology, cosmetics, medicine. The re- active functional groups of chitosan include an amino group and both primary and secondary hydroxyl groups at C-2, C-3, and C-6 positions, respectively. The biological activities of chitosan and its derivatives correlated with their structures and physicochemical properties [11, 12]. The green synthesized nanoparticles such as silver, selenium, zinc oxide and copper etc. shows very good an- tioxidant, antimicrobial and many more biomedical applications [13-20]. Abstract Nanoparticles and nano-composites are major advanced materials used in many medical and dental applications. In this pre- sent investigation, we have prepared silymarin, chitosan and hydroxyapatite based nanocomposite using magnetic stirring. The prepared nanocomposite characterized using UV-vis spectroscopic analysis. The free radical scavenging activity of prepared nanocomposites was tested using DPPH assay. The results are statically analyzed and the antioxidant results are good for na- nocomposite materials. Based on the results the prepred nanocomposites may used in various dental applications. Keywords: Nanocomposites; Antioxidant; Chitosan; Silymarin; HAP.