Nikita Sivakumar, R. V. Geetha, Vishnu Priya, Gayathri R, Dhanraj Ganapathy. Targeted Phytotherapy For Reactive Oxygen Species Linked Oral Cancer. Int J Dentistry Oral Sci. 2021;8(1):1425- 1429. 1425 OPEN ACCESS https://scidoc.org/IJDOS.php Targeted Phytotherapy For Reactive Oxygen Species Linked Oral Cancer Research Article Nikita Sivakumar 1 , R. V. Geetha 2 , Vishnu Priya 3 , Gayathri R 4 , Dhanraj Ganapathy 5* 1 Intern, Department of Prosthodontics, Saveetha Dental College and Hospitals, Chennai - 600 077, India. 2 Assistant Professor, Department of Microbiology, Saveetha Dental College and Hospitals, Chennai - 600 077, India. 3 Professor and Head, Department of Biochemistry, Saveetha Dental College And Hospitals, Saveetha Institute Of Medical And Technical Sciences Chennai, India. 4 Reader, Department of Physiology, Saveetha Dental College and Hospitals, Chennai - 600 077, India. 5 Professor and Head, Department of Prosthodontics, Saveetha Dental College And Hospitals, Saveetha Institute Of Medical And Technical Sciences Chennai, India. International Journal of Dentistry and Oral Science (IJDOS) ISSN: 2377-8075 *Corresponding Author: Dhanraj Ganapathy, Professor and Head, Department of Prosthodontics, Saveetha Dental College,Chennai, 600 077, India. E-mail: dhanraj@saveetha.com Received: January 12, 2021 Accepted: January 22, 2021 Published: January 28, 2021 Citation: Nikita Sivakumar, R. V. Geetha, Vishnu Priya, Gayathri R, Dhanraj Ganapathy. Targeted Phytotherapy For Reactive Oxygen Species Linked Oral Cancer. Int J Dentistry Oral Sci. 2021;8(1):1425-1429. doi: http://dx.doi.org/10.19070/2377-8075-21000284 Copyright: Dhanraj Ganapathy © 2021. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. Introduction The great advances in medicine for synthetic drugs, might have made us overlook the healing power of a “natural” source; the plants [14]. However, since the consumer’s interest is gradually shifting towards the more “natural” and “safer” alternative, Phy- totherapy has once again come back to its peak of research [8]. In this research, two plant extracts will be used; Cassia fstula and Cassia alata. They belong to the same family, Fabaceae [4]. Cassia alata which is also known as Sennaalata, is a medicinal plant used as vermicide, astringent, purgative, expectorant and to treat skin diseases. It has demonstrated effective broad spectrum antibac- terial, anticandidal, and antifungal activities in several laboratory studies over the years [12]. It also relieves pain, reduces infamma- tion, helps in digestion and repels insects. The parts commonly used for medicinal purposes are the leaves and the bark [18, 6]. On the other hand, Cassia Fistula also has various medicinal uses. It is commonly known as Amaltas. They are native to South-East Asia and generally found in the Himalayas [20]. Cassia Fistula true in a whole is very useful; the root bark contains tannin, phlobap- henes and oxy anthraquinone, the pulp containing rhein, a volatile oil and a resinous substance and notably the fruits of cassia fstula are used in the Indian Pharmaceutical codex. Cassia Fistula ex- tracts are commonly used to treat Aguesia, skin disorders, intesti- nal disorders, common cold and constipation [1]. Reactive oxygen species (ROS) may be stimulated by oxidative stress, nutrient starvation, mitochondrial toxins and hypoxia which eventually induces carcinogenesis [2]. In this research, two plant extracts will be used to study its phytochemical analysis and its role in phytotherapy for reactive oxygen species linked to oral Abstract Medicinal plants play a major role in medical and health needs. In developing countries, there is an increasing attempt to incor- porate the traditional medicines, especially herbal preparations in the local healthcare systems and people are increasingly turn- ing to herbal medicine. Plant derived therapies are suggested for its cost effectiveness, fewer side effects and lesser frequencies of resistance. The aim of this study is to understand the free radical scavenging properties of selected Indian medicinal plants and their cytotoxicity against oral cancer cell lines.The plant parts were shade dried, powdered and extracted using methanol solvent. These extracts were analyzed for the free radical scavenging properties in Cell lines by Nitric oxide assay. Further, an MTT assay was performed to understand the cytotoxicity of the extracts. Data showed a signifcant percentage of inhibition of the free radicals and cytotoxicity against the oral cancer cell lines.Selected plants have been shown to possess inhibitory potentials against ROS-linked oral cancers and may be used for targeted phytotherapy. Keywords: Phytotherapy; Herbal; ROS; Cytotoxicity; Cancer; Free Radicals.