International Journal of Engineering Applied Sciences and Technology, 2019 Vol. 3, Issue 12, ISSN No. 2455-2143, Pages 85-87 Published Online April 2019 in IJEAST (http://www.ijeast.com) 85 EFFECT OF VOLTAGE IN ELECTRO- HYDROLYTIC PRE-TREATMENT ON ANAEROBIC DIGESTION PERIOD Shubham Bhatt Student, Dept of Biotechnology, UCALS, Uttaranchal University, Dehradun Saurabh Nishad Student, Dept of Biotechnology, UCALS, Uttaranchal University, Dehradun Utkarsh Singh Student, Dept of Biotechnology, UCALS, Uttaranchal University, Dehradun , Dr. Rohit Sharma Assistant Professor, Dept of Biotechnology, UCALS, Uttaranchal University, Dehradun Abstract— Anaerobic digestion is well known and well developed technology applied for bioenergy production from different types of feedstock like animal manure, sewage sludge, agricultural residue, industrial waste etc. These different types of substrate are having high content of biodegradable material or organics which make this process an ideal biomass for anaerobic digestion process. Due to the natural intractable structure of lignocellulose biomass towards microbial or enzymatic deconstruction, pre-treatment is necessary to make the celluloses open to enzymatic hydrolysis for fermentation and anaerobic digestions. In recent years, there has been improved concern in pretreatment of lignocellulose biomass and waste for anaerobic digestion. This study was also focussed on the pre-treatment of lignocellosic biomass. In this research, the yield of biogas at 50 V was found to be 90.4 %, 100 V was 83.1 respectively; whereas, without electro-hydrolysis 76.2 % was observed. Moreover, the pretreated biomass gets stabilized at 15th day, but it took more than 18 days for the control or untreated biomass to get stabilize. Keywords— Anaerobic digestion, lignocellulose, Electro- hydrolytic, Rice Husk I. INTRODUCTION An anaerobic digestion is well developed technology widely applied for bioenergy production from the sewage sludge, animal manure, agricultural residue, industrial sludge etc. This substrate has high levels of biodegradable organics make it an ideal feedstock for anaerobic digestion. Due to the continuous production in the industrial level, there is a huge accumulation of waste especially in sugar mills; the huge quantity of bagasse as residue was produced. The lignocellulose biomass is mainly consists of cellulose, hemicellulose, and lignin. Due to the natural intractable structure of lignocellulose biomass towards microbial or enzymatic deconstruction, pre-treatment is necessary to make the celluloses open to enzymatic hydrolysis for fermentation and anaerobic digestions. In recent years, there has been improved concern in pretreatment of lignocellulose biomass and waste for anaerobic digestion, Elliot et al. (2007). The several structural and compositional properties of lignocellulosic content render it resistant to biodegradation. Even though there has been an extensive research on the effect of pretreatment, the correlation between the degradability of lignocellulose material, the structural and compositional properties remains unclear and contradictory. Use of lignocellulosic biomass for value-added products Lignocellulosic biomass is having potential as renewable resource which is present in plant cell and can be utilize directly for the production of energy products which can be used for a various purposes. The effect of hydrolysis of lignocellulosic biomass is shown in Figure 1. Fig. 1. Effect of hydrolysis of lignocellulosic biomass [2] But, it is essential to break the three dimensional structure of polymer which is present in biomass into the simpler composites for gaining optimized advantage in various form of valuable products. The different forms of value added products from lignocellulosic biomass are shown in Figure 5. Elliot et al. (2012) and Hu et al. (2016) concluded that hydrolysis of biomass is the rate-limiting stage during