622 THIRD-PARTY T CELL IMMUNOTHERAPY FOR VIRAL INFECTIONS IN PRIMARY IMMUNODEFICIENCY DISORDERS Michael D. Keller, MD 1 , Patrick J. Hanley, PhD 2 , Jamie Hoover, BA 2 , Lauren Roesch, BA 2 , Sarah McCormack, BS 2 , Haili Lang, MS 2 , Allistair Abraham, MD 2 , Kirsten Williams, MD 2 , Blachy Davila, MD 2 , Roberta Adams, MD 3 , Jennifer W. Leiding, MD, FAAAAI 4 , and Catherine Bollard, MD 2 ; 1 Allergy and Immunology, Children’s National Medical Center, Washington, DC, 2 Children’s National Medical Center, Washing- ton, DC, 3 Phoenix Children’s, Phoenix, AZ, 4 University of South Florida Morsani College of Medicine, St. Petersburg, FL. RATIONALE: Adoptive immunotherapy using virus-specific T-lympho- cytes (VSTs) has been successful in treating viral infections after hematopoietic stem cell transplantation (HSCT). Use of partially HLA- matched VSTs from third-party donors allow ‘‘off the shelf’’ therapy for viral infections. We hypothesize that third-party VSTwill be effective and safe in patients with primary immunodeficiency disorders (PID). METHODS: VSTs were cultured from healthy donors via viral pepmix stimulation and expansion. Patients received third party VST infusion before or after HSCT for treatment of CMV, EBV, or adenovirus. Antiviral immunity was assessed via IFNg ELISpot, IFNg capture flow cytometry, and TCRb sequencing. Patients were followed for 45 days following infusion for toxicities and for up to 12 months for antiviral immune reconstitution. RESULTS: Seven patients with PID received 9 VST products at a dose of 2 x 10E7 cells/m2. Four patients had undergone HSCT, while three patients were treated before HSCT due to persistent viral infections. None of the patients developed GVHD, though transient flaring of viral hepatitis occurred in two patients after infusion. Antiviral responses were seen in 5 of 7 patients. Of the pre-HSCT patients, two infants with SCID and cidofovir-refractory adenovirus each received 2 VST infusions and cleared their infections. Expansion of virus-specific T-cells was detectable by IFNg ELIspot or gamma capture in 3 of 5 responding patients. VST persistence was also confirmed by TCRb sequencing in one patient. CONCLUSIONS: Third-party VST are safe in patients with PID, and may be a valuable salvage therapy for the treatment of viral infections before or after HSCT. 623 Regulatory Variants of ATF3, CDH17 and FAM71A are Risk Factors for Diisocyanate Induced Occupational Asthma (DA) Zana L. Lummus, PhD 1 , Banu Kesavalu, MS 1 , Kenneth Kaufman, PhD 2 , Jianbo Yao, PhD 3 , Matthew T. Weirauch, PhD 2 , Leah C. Kottyan, PhD 2 , Daniel Miller, BS 2 , Andr e Cartier, MD, FAAAAI 4 , Maria-Jesus Cruz, MD 5 , Catherine Lemiere, MD 4 , Xavier Mu~ noz, MD 5 , Santiago Quirce, MD, PhD 6 , Joaquin Sastre, MD, PhD, FAAAAI 7 , Susan M. Tarlo, MB, BS, FAAAAI 8 , and David I. Bernstein, MD, FAAAAI 9 ; 1 Uni- versity of Cincinnati College of Medicine, Cincinnati, OH, 2 Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, 3 West Virginia Uni- versity, Morgantown, WV, 4 Universit e de Montr eal, Montr eal, QC, Can- ada, 5 Hospital Universitari Vall d’Hebron, Barcelona, Spain, 6 Hospital La Paz, Madrid, Spain, 7 Fundacion Jimenez Diaz, Madrid, Spain, 8 Univer- sity of Toronto, Toronto, ON, Canada, 9 University of Cincinnati, Cincin- nati, OH. RATIONALE: Using Next Generation Sequencing (NGS) we identified non-coding SNPs associated with confirmed DA that might impact gene expression via transcription factor (TF) binding interactions. METHODS: DNA was collected from 91 diisocyanate exposed workers with confirmed DA. Fourteen loci containing DA associated SNPs were sequenced and compared to 293 subjects from the 1,000 genomic (1KG) data set. 22 top-ranked SNPs associated with DA (X 2 p< 1 x 10 -3 ) under- went transcriptomic analysis. Electrophoretic mobility shift assays (EMSA) identified oligonucleotide-protein binding for risk and non-risk SNPs to nuclear extracts of A549, BEAS 2B, and IMR-90 lung cell lines. Oligonucleotide bound proteins were purified by DNA affinity precipita- tion assays (DAPA) and eluted proteins were identified by mass spectrom- etry (MS). A luciferase reporter assay, in A549 cells transfected with luciferase reporter constructs, identified allele-dependent mRNA transcription. RESULTS: The 22 top-ranked SNPs identified in non-coding regions included: CDH17 (10), ATF3 (7), FAM71A (2), PITPNC1, TACR1, ZBTB16. EMSA detected A549 nuclear protein binding to 10 of the 22 variants, with 8 displaying preferential binding to non-risk alleles (rs1001304, rs2287231,rs2513789, rs2513791, rs11571537, rs14798008) or risk alleles (rs2513788, rs2513790). MS revealed rs14798008 bound to H1 histones. Four SNPs exhibited allele-dependent increases in gene expression (rs2287231, rs2513789, rs11571537 and rs2446824). CONCLUSIONS: We identified 5 potential regulatory allele-dependent SNPs for DA. Four variants exhibited functional activity for dysregulation of gene transcription. The fifth variant, in the promoter region of FAM71A (rs14798008), showed non-risk allele preferential binding to H1 histones, that modulate chromosomal accessibility of regulatory proteins to DNA. 624 A Role for B Cells in Organic Dust Induced Lung Injury Jill A. Poole, MD 1 , Ted R. Mikuls 2 , Michael J. Duryee 2 , Kristi J. J. Warren, PhD 3 , Todd A. Wyatt, PhD 2 , Amy J. Nelson 2 , Debra J. Rom- berger 2 , William W. West, MD 2 , and Geoffrey Thiele, PhD 2 ; 1 University of Nebraska Medical Center, Omaha, NE, 2 UNMC, Omaha, NE, 3 Internal Medicine - Pulmonary, Critical Care, Sleep and Allergy, University of Ne- braska Medicine, Omaha, NE. RATIONALE: Agriculture organic dust exposures induce lung disease with lymphoid aggregates comprised of both T and B cells. The precise role of B cells in mediating lung injury is unknown, yet might be relevant given the emerging role of B cells in obstructive airways disease and associated autoimmunity. METHODS: Using an established intranasal inhalation exposure model, C57BL/6 wild-type (WT) and B-cell receptor (BCR) knock-out (KO) mice were treated daily with saline or swine confinement organic dust extract (ODE) for 3 weeks. Bronchoalveolar lavage fluids, lung tissues, and serum were collected. Cytokines analyzed by ELISA; cellular influx by flow cytometry. Serum collected to measure immunoglobulins and autoanti- body responses. Lung tissues stained for modified self-proteins. RESULTS: ODE-induced neutrophil influx was not reduced in BCR KO animals, but there was reduction in TNF-a, IL-6, CXCL1, and CXCL2 release. ODE-induced lymphoid aggregates, failed to develop in BCR KO mice. Compared to saline, there was an expansion of conventional B2-, innate B1 (CD19 + CD11b + CD5 +/- )-, and memory (CD19 + CD273 +/- CD73 +/- ) B cells following ODE exposure in WT mice. Serum IgG responses, anti-citrullinated protein antibody (ACPA), and anti-malondialdehyde-acetaldehyde (MAA) autoantibody responses were increased in ODE treated WT mice vs. saline control. B cells and serum immunoglobulins were not detected in BCR KO animals. Lung tissue staining for citrullinated and MAA modified proteins were increased in ODE treated WT animals, but not BCR KO mice. CONCLUSIONS: Agriculture organic dust induced lung injury is dependent upon B cells, and this exposure induces autoreactive immune responses to citrullinated and MAA-modified proteins implicated in the systemic autoimmune pathogenesis. J ALLERGY CLIN IMMUNOL FEBRUARY 2018 AB198 Abstracts SUNDAY