International Journal of Agricultural Science Research Vol. 2(6), pp. 206-213, June 2013
Available online at http://academeresearchjournals.org/journal/ijasr
ISSN 2327-3321 ©2013 Academe Research Journals
Full Length Research Paper
Effect of plant chemicals on oviposition deterrent and
ovicidal activities against female moth, Spodoptera
litura (Fab.) (Lepidoptera: Noctuidae)
A. Jeyasankar
1
*, K. Elumalai
2
, N. Raja
3
and S. Ignacimuthu
4
1
Department of Zoology, Arignar Anna Government Arts College, Musuri - 621 211, Tamilnadu, India.
2
Unit of Entomotoxicity, Department of Advanced Zoology and Biotechnology, Govt. Arts College Nandanam, Chennai -
600 035, Tamil Nadu, India.
3
Department of Biology, Faculty of Natural and Computational Sciences, Post Box 196, University of Gondar, Ethiopia.
4
Entomology Research Insitutue, Loyola College, Chennai-600 034, Tamilnadu, India.
Accepted 21 May, 2013
Oviposition deterrent and ovicidal activity of crude extracts, fractions and compounds A and B from the
leaves of Syzigium lineare Wall., were tested against armyworm Spodoptera litura Fab. The
concentration tested for crude extract was 0.625, 1.25, 2.50 and 5.0%; fractions of 0.025, 0.05, 0.075 and
0.1%; and compounds A and B of 25, 50, 75 and 100 ppm, respectively. Among the crude extracts
tested, the maximum oviposition deterrent and ovicidal activity were observed in ethyl acetate extract at
5% concentration. In fractionation, maximum oviposition deterrent was found in fraction 6 and ovicidal
activity was the highest in fraction 3. Both activities were statistically significant by LSD “(p<0.05)
compared” to positive and negative control. Compounds A and B were tested for oviposition deterrent
and ovicidal activities of S. litura. Highest oviposition deterrent was noticed in compound A and
significant ovicidal activity was found in compound B compared to the control. Based on IR,
1
H and
13
C
NMR data, compound A was 7-hydroxy-undec-1en-3-one and compound B was 3-(3-hydroxy-hexyl)-
tetrahydro-pyran-4-one. The oviposition deterrent and ovicidal activity of S. lineare reported against S.
litura was the first of its kind and it can be useful for the development of novel botanical pesticides in
future after field confirmation.
Key words: Oviposition deterrent, ovicidal, Syzigium lineare, Spodoptera litura, plant extracts.
INTRODUCTION
Spodoptera litura Fab. (Lepidoptera: Noctuidae) is
commonly called army worm which is an economically
important pest of cosmopolitan distribution, and it causes
extensive damage to more than 180 crops (Isman et al.,
2007). The damage caused by the pests is mainly by
defoliation and the estimated damage potential in
tobacco nurseries is to an extent of 80 to 100% and 72 to
98% in soybean (Chari and Ramaprasad, 1987; Singh,
2000). Due to growing awareness about the negative
impact of chemical pesticides, it becomes imperative to
develop alternative strategies. One of the safer
alternative methods is using locally available plant
resource in a sustainable way. The plant extracts with
oviposition deterrent and ovicidal activity are really a
valuable weapon to protect the crops before the plants
were damaged by the pests. Statistically significant
feeding deterrent activity was observed in ethyl acetate
extract of Hydrophila auriculata at 5% concentration
against S. litura (Basker et al., 2011). In a cage study,
crude oil emulsion of neem seed extract reduced egg
laying properties of S. litura on treated cabbage plant
(Naumann and Isman, 1995). Raja et al. (2004) reported
oviposition deterrent activities of hexane extract of Aegle
*Corresponding author. E-mail: sankar.alagarmalai@gmail.com.
Tel: +91 9894214939.