3972 INTRODUCTION The metabolic and mechanical requirements of human walking influence a broad array of structural, functional and health relationships. This global functional importance has stimulated a body of scientific literature that now spans more than a century and encompasses a variety of experimental objectives. These range from basic biological inquiry to applied efforts to predict speed, energy expenditure and other variables in laboratory and field settings. However, in spite of the extensive scientific consideration that human walking has received, some aspects of basic understanding remain limited. A primary example of incomplete contemporary understanding is the body size dependency long observed for the metabolic requirements of this gait. As would be expected, larger individuals do expend more energy than smaller ones when the metabolic energy expended is expressed in absolute terms. However, the differences observed are not directly proportional to body mass. When expressed on a per kilogram basis, the energy expended to walk a fixed distance or at a given speed can be as much as two to three times greater for smaller versus larger individuals. At present, a quantitative explanation for the relationship between body size and the energy cost of human walking has not been established. The greater mass-specific metabolic rates consistently observed for smaller versus larger human walkers have been considered from several perspectives. Ontogenetic approaches have appropriately considered both maturation (DeJaeger et al., 2001; Morgan et al., 2002) and body size (McCann and Adams, 2002) but have not resolved their quantitative importance. Mechanical approaches have estimated that the mass-specific mechanical work that small children and adults perform during walking differs only marginally (Cavagna et al., 1983; Bastien et al., 2003; Schepens et al., 2004) and therefore does not account (Schepens et al., 2004) for the much larger differences observed in metabolic cost. The current lack of quantitative understanding is reflected in the use of different generalized equations to estimate the energy expended by adult (ACSM, 2006; Pandolf et al., 1971) and child populations (Morgan et al., 2002). In both cases, population-specific equations predict the same mass-specific metabolic rates for individuals who differ in height and mass. A potential explanation for the apparent body-size dependency of the metabolic cost of human walking is a corresponding rate dependency in executing the mechanics of each walking stride (Alexander, 1976; Heglund and Taylor, 1988). Clearly, the shorter statures of smaller versus larger walkers require more, and more frequent, strides in order to travel any fixed distance or at any given speed. If the mechanical components of each walking stride were to require the same expenditure of metabolic energy per kilogram of body mass, shorter walkers might have greater mass-specific metabolic rates simply because they take more frequent strides. This possibility seems most plausible if shorter and taller individuals were to walk in dynamically similar ways, i.e. with both stride lengths and times related to the body’s length (L b ) by some constant proportion. Although widely embraced (DeJaeger et al., 2001; McCann and Adams, 2002; Cavagna et al., 1983), the validity of the dynamic similarity assumption is not strictly known. Thus, the simple possibility that the energy cost per stride at equivalent speeds may be the same for short and tall individuals has not been evaluated. The Journal of Experimental Biology 213, 3972-3979 © 2010. Published by The Company of Biologists Ltd doi:10.1242/jeb.048199 The mass-specific energy cost of human walking is set by stature Peter G. Weyand 1, *, Bethany R. Smith 2 , Maurice R. Puyau 3 and Nancy F. Butte 3 1 Locomotor Performance Laboratory, Department of Applied Physiology and Wellness, Southern Methodist University, Dallas, TX 75205, USA, 2 Locomotion Laboratory, Department of Kinesiology, Rice University, Houston, TX 77005, USA and 3 USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA *Author for correspondence (pweyand@smu.edu) Accepted 6 September 2010 SUMMARY The metabolic and mechanical requirements of walking are considered to be of fundamental importance to the health, physiological function and even the evolution of modern humans. Although walking energy expenditure and gait mechanics are clearly linked, a direct quantitative relationship has not emerged in more than a century of formal investigation. Here, on the basis of previous observations that children and smaller adult walkers expend more energy on a per kilogram basis than larger ones do, and the theory of dynamic similarity, we hypothesized that body length (or stature, L b ) explains the apparent body-size dependency of human walking economy. We measured metabolic rates and gait mechanics at six speeds from 0.4 to 1.9 m s –1 in 48 human subjects who varied by a factor of 1.5 in stature and approximately six in both age and body mass. In accordance with theoretical expectation, we found the most economical walking speeds measured (J kg –1 m –1 ) to be dynamically equivalent (i.e. similar U, where Uvelocity 2 /gravity · leg length) among smaller and larger individuals. At these speeds, stride lengths were directly proportional to stature whereas the metabolic cost per stride was largely invariant (2.74±0.12 J kg –1 stride –1 ). The tight coupling of stature, gait mechanics and metabolic energy expenditure resulted in an inverse relationship between mass-specific transport costs and stature (E trans /M b L b –0.95 , J kg –1 m –1 ). We conclude that humans spanning a broad range of ages, statures and masses incur the same mass-specific metabolic cost to walk a horizontal distance equal to their stature. Supplementary material available online at http://jeb.biologists.org/cgi/content/full/213/23/3972/DC1 Key words: metabolism, scaling, locomotion, biomechanics. THE฀JOURNAL฀OF฀EXPERIMENTAL฀BIOLOGY