Basic Sciences of Medicine 2014, 3(2): 26-29
DOI: 10.5923/j.medicine.20140302.02
Anti-hepatitis C virus (HCV) Antibody Detection among
Fresh Undergraduate Students in Port Harcourt, Nigeria
Okonko I. O.
1,*
, Ikunga C. V.
1
, Anugweje K. C.
2
, Okerentugba P. O.
1
1
Department of Microbiology, University of Port Harcourt, Choba, East-West Road, P.M.B. 5323, Choba, Port Harcourt,
Rivers State, Nigeria
2
Department of Health Services, University of Port Harcourt, Choba, East-West Road, P.M.B. 5323, Choba, Port Harcourt,
Rivers State, Nigeria
Abstract This study reports carried out to detect the presence or absence of anti-HCV antibody among fresh
undergraduate students in Port Harcourt, Nigeria. The subjects included in this study were 200 [(100(50%)] males and
[(100(50%)] females; of ages between 16–20 years, 21–25 years, and 26 years and above. Plasma samples from the subjects
were tested for the presence of antibodies to HCV using Global® HCV-Ab kit (Global Diagnostics, USA)and ACON
HCV-Ab kit (ACON Laboratories, Inc. San Diego, USA) in a stepwise order for the detection of anti-HCV antibodies. The
overall prevalence rate was 0.0%. Also age groups 16–20 years, 21–25 years, and 26 years and above had the same
prevalence rate of 0.0%. The prevalence rate of HCV antibody was the same among males and their female counterparts with
the rate of 0.0%. This study however, revealed the absence of this infection among the fresh undergraduate students
population in Port Harcourt, Nigeria. Further studies on a larger scale is advocated for the generation of data and the use of
rapid test kit should be used in conjunction with other immunoassay particularly Elisa technique are therefore recommended.
Keywords Antibodies, HCV, Undergraduate students, Nigeria
1. Introduction
Hepatitis is an inflammatory condition of the liver while
viral hepatitis is a conventional term used to denote hepatitis
caused by the hepatotrophic viruses (hepatitis A-G) [1].After
its discovery and characterization by Choo et al [2], hepatitis
C Virus has remained a major cause of chronic liver disease
worldwide and the main reason for liver transplantation in
the western world [3]. HCV infections are known to occur in
the general population.
Approximately 170 million people worldwide who are
about 3-4% of the world population are chronically infected
with the hepatitis C virus who are at risk of developing liver
cirrhosis, cancer or both has been shown to have a worldwide
distribution, occurring among persons of all ages, genders,
races and regions of the world [4]. Slightly different
prevalence was reported from different regions of the world.
Prevalence of 1.7% was reported from America, 1.03% from
Europe, 3.9% from the Western Pacific, 4.6% from the
Eastern Mediterranean, 2.15% from South Asia and 5.3%
from Africa [5-7].
Among HCV infected persons only 20-30% has
* Corresponding author:
mac2finney@yahoo.com (Okonko I. O.)
Published online at http://journal.sapub.org/medicine
Copyright © 2014 Scientific & Academic Publishing. All Rights Reserved
symptoms of acute hepatitis. About 75%-85% of infected
older adults and 50-60% of infected Juveniles or young
adults become chronically infected. Majority of persons with
chronic HCV infections are asymptomatic [8].
Currently, new HCV infections are primarily due to
intravenous or nasal drug use, and to a lesser degree to unsafe
medical or surgical procedures. Parenteral transmission via
tattooing or acupuncture with unsafe materials is also
implicated in occasional transmissions. The risk of perinatal
and of heterosexual transmission is low, while recent data
indicate that promiscuous male homosexual activity is
related to HCV infection [9].
The factors that determine the outcome and natural course
of HCV infection are not completely understood. However,
it is generally accepted that next to virological factors innate
and adaptive immune responses play an important role in
both, control of HCV infection and as disease pathogenesis
[10]. HCV replicates mainly in hepatocytes, but its nucleic
acids have also been found in peripheral blood mononuclear
cells and in central nervous system cells [11].
The detection of anti-HCV antibodies in plasma or serum
is based on serological tests especially the use of enzyme
immunoassays (EIAs) or Enzyme Linked Immunosorbent
assays (ELISA) which are now commercially available[6]
and molecular (PCR being used to determine the extent of
the virus variation) [12]. This study reports carried out to
detect the presence or absence of anti-HCV antibody among